摘要: |
目的:观察三唑醇类化合物SL-130的体内外抗真菌活性。 方法:采用美国临床实验室标准化委员会(NCCLS)1997年公布的M27-A方案,实验用菌株包括白念珠菌及非念珠菌致病真菌,用最低抑菌浓度MIC80表示抗真菌活性。此外利用纸片扩散法、时间-杀菌曲线及小鼠的存活率等方法考察SL-130的抗真菌活性。结果:SL-130具有一定的抗真菌活性,体外明显强于氟康唑,体内与氟康唑基本相当。结论:此三唑醇类化合物结构新颖,体内外的抗真菌活性明显,值得进一步研究。 |
关键词: 三唑类;抗真菌药 微生物敏感性试验 SL-130 |
DOI:10.3724/SP.J.1008.2008.00813 |
投稿时间:2008-03-05修订日期:2008-04-29 |
基金项目:国家重点基础研究发展计划 (“973”计划,2005CB523105),国家自然科学基金(30300437). |
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In vitro and in vivo antifungal activity of a triadimenol compound |
OUYANG Xiao-fang,CAO Yong-bing,SUN Qing-yan,WANG Yan,JIANG Yuan-ying* |
(Department of Pharmacology,School of Pharmacy,Second Military Medical University,Shanghai 200433,China) |
Abstract: |
Objective:To observe the in vitro and in vivo antifungal activity of a triadimenol compound SL-130.Methods: The M27-A project recommended by the National Committee for Clinical Laboratory Standard (NCCLS) in 1997 was used in the present study.The minimum inhibitory concentrations (MIC80) of SL-130 were determined for candidal and non-candidal strains with different susceptibilities to fluconazole.Agar disk diffusion test,time-kill curves test and mouse survival rate were employed to examine the antifungal activity of SL-130.Results: The results indicated that SL-130 had stronger in vitro antifungal activity than fluconazole,and similar in vivo activity to fluconazole.Conclusion:The structure of SL-130 is quite unique and it has in vitro and in vivo antifungal activity; further study is warranted on the compound. |
Key words: triazoles antifungal agents microbial sensitivity tests SL-130 |