| 摘要: |
| 目的:探讨TNF-A-863与CGRP979基因多态性与重度慢性牙周炎易感性的关系。方法:收集100例重度慢性牙周炎患者和118例健康对照组的颊黏膜拭子并抽提DNA，应用PCR-LDR方法检测TNF-A-863与CGRP979基因型,用计算机软件统计分析患者和对照组间基因型分布的差异。结果:TNF-A-863在重度慢性牙周炎组中TNF-A-863“A/C”杂合子占优势，在健康对照组中TNF-A-863“C/C”纯合子基因型为主，TNF-A-863基因型分布在两组间的差异有统计学意义(P<0.05)。CGRP979在重度慢性牙周炎患者中“A/G”基因型明显占多数，两组间基因型分布有统计学意义(P<0.05)。结论:TNF-A-863基因多态性与重度慢性牙周炎有相关性； CGRP979等位基因“A/G”杂合子基因型可能是重度牙周炎的易感因素。 |
| 关键词: 重度慢性牙周炎 肿瘤坏死因子A-863 降钙素基因相关肽 基因多态性 |
| DOI：10.3724/SP.J.1008.2009.0541 |
| 投稿时间：2008-03-16修订日期：2009-03-12 |
| 基金项目:上海市自然科学基金（06ZR14123），全军“十一五”面上项目（06ZR14123）. |
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| Association of TNF-A-863 and CGRP979 gene polymorphisms with susceptibility to severe chronic periodontitis in Chinese Han nationality |
| LI Ling-min，CAO Zhi-zhong* |
| (Department of Stomatology,Changhai Hospital，Second Military Medical University，Shanghai 200433，China) |
| Abstract: |
| Objective:To investigate the relationship of TNF-A-863 and CGRP979 gene polymorphisms with the susceptibility to severe chronic periodontitis in Chinese.Methods: Buccal swabs were collected from 100 adult patients with severe chronic periodontitis and 118 healthy adult controls.DNA was extracted from each subjects of the two groups.PCR-LDR technique was used to identify the genotypes of TNF-A-863 and CGRP979.The difference in the genotypes between the two groups was analyzed by statistics software.Results: The genotype of TNF-A-863 was mainly TNF-A-863 A/C in patients with severe chronic periodontitis and TNF-A-863 C/C in healthy controls.There were significant differences in TNF-A-863 distribution between the two groups(P<0.05).We also found that there were significant differences in genotype distribution of CGRP979 between the two groups (P<0.05),with A/G predominating in patients with severe chronic periodontitis.Conclusion: TNF-A-863 polymorphism is associated with severe chronic periodontitis; A/G of the CGRP979 loci might be a factor for severe chronic periodontitis. |
| Key words: severe chronic periodontitis tumour necrosis factor A-863 calcitonin gene-related peptide gene polymorphisms |
