| 摘要: |
| 目的:研究脑红蛋白(neuroglobin,Ngb)在大鼠脑缺血模型中的动态表达及缺血缺氧后Ngb的脑保护作用。方法:将SD大鼠随机分为4组,即大脑中动脉闭塞(middle cerebral artery occlusion,MCAO)组、出血性脑梗死(HI)组、氯化血红素(Hemin)干预组和假手术组,以建模后3、6、12、24 h为观察点,测定脑组织含水量、脑梗死体积,H-E染色观察组织病理学改变,免疫组化染色观察Ngb的表达。结果:Hemin处理组脑组织含水量、脑梗死体积较MCAO组和HI组有显著性差异(P<0.01)；HI组脑水肿在12 h显著升高；病理切片观察,Hemin处理组水肿程度及变性神经元数量较MCAO组和HI组减轻；免疫组化分析,Hemin处理组Ngb阳性神经元数量较MCAO组和HI组增多。结论:HI组脑水肿高峰提前可能导致原有病情的加重,应用Hemin诱导Ngb的表达,可减轻局灶性脑缺血时脑组织的损伤,从而达到脑保护作用。 |
| 关键词: 大脑中动脉梗死 出血性脑梗死 氯化血红素 脑红蛋白 |
| DOI：10.3724/SP.J.1008.2008.00 |
| 投稿时间：2008-03-22修订日期：2008-05-24 |
| 基金项目:河北省自然科学基金(C2006000915). |
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| Expression of neuroglobin after focal cerebral ischemia and its neuroprotective effect |
| YIN Jing, ZHANG Xiang-jian*, LI Li-tao, YANG Yi |
| (Department of Neurology, Second Affiliated Hospital of Hebei Medical University, Shijiazhuang 050000, China) |
| Abstract: |
| Objective:To study the expression of neuroglobin (Ngb) in rat cerebral ischemia model and the neuroprotective effect of Ngb after ischemia and hypoxia. Methods: Totally 113 Sprague-Dawley rats were randomly divided into sham-operated group, middle cerebral artery occlusion (MCAO) group, hemorrhagic infarction (HI) group and hemin treatment group. The brain water content, infarcted tissue volume, neuropathologic changes (H-E staining) and expression of Ngb (immunocytochemical staining) were examined 3, 6, 12, and 24 h after model establishment. Results: The brain water contents and the infarcted tissue volumes in the hemin treatment group were significantly different from those of the MCAO group and HI group (P<0.01). The brain edema was obviously increased in HI group at 12 h. Neuropathologic examination showed that there were fewer necrotic neurons, milder edema and stronger Ngb expression in the Hemin treatment group than in the MCAO group and HI group. Immunocytochemical staining showed that the Ngb positive neurons in Hemin treatment group were more than those in the MCAO and HI groups. Conclusion: Earlier peak of brain edema may lead to aggravation of disease. Hemin-induced Ngb expression may relieve brain damage during focal cerebral ischemia. |
| Key words: middle cerebral artery infarction hemorrhagic infarction Hemin neuroglobin |
