| 摘要: |
| 目的:观察不同降温速率下血小板冰冻保存效果，探讨血小板冰冻保存的最佳降温速率。方法:在新型血小板冷冻保护剂作用下，血小板以不同速率(1、10、20℃/min)降温至-80℃后直接转入深低温冰箱保存，冻存1周后复温检测其体外功能和表面分子的变化，与非程序降温组及新鲜血小板作比较。结果:复温后各冰冻保存组的血小板计数均低于新鲜对照组(P<0.05或P<0.01)。肾上腺素诱导的最大聚集率10℃/min组与1℃/min组、非程序降温组两两比较无统计学差异，低于新鲜对照组(P<0.01)，但高于20℃/min组(P<0.05)。CD42b表达的平均荧光强度10℃/min组高于其他各冷冻保存组(P<0.05)，低于新鲜对照组，但无统计学意义。血小板复温后1℃/min组、20℃/min组、非程序降温组表面的CD62p荧光强度均高于新鲜对照组(P<0.05)，10℃/min组CD62p荧光强度高于新鲜对照组，但无统计学差异。结论:降温速率对冰冻保存血小板效果影响较大，在新型血小板冷冻保护剂作用下，相对于1、20℃/min，10℃/min降温速率效果最佳。 |
| 关键词: 低温保存 血小板 血小板计数 CD41抗原 CD42b抗原 CD62p抗原 |
| DOI：10.3724/SP.J.1008.2008.00809 |
| 投稿时间：2008-04-20修订日期：2008-07-01 |
| 基金项目:军队“十一五”课题(06MA180). |
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| Influence of cooling rate on quality of cryopreservted platelets |
| HU Hui-hong1,QIAN Bao-hua1*,LIU Bao-lin2,GU Hai-hui1,ZHU Yan-xia1,HUA Mei-xian1 |
| (1.Department of Blood Transfusion,Changhai Hospital,Second Military Medical University,Shanghai 200433,China;2.Biotechnology Institute of Low Temperature,University of Shanghai for Science and Technology,Shanghai 200093,China) |
| Abstract: |
| Objective:To observe the outcomes of different cooling rates in the cryopreservation of platelets,so as to screen the best cooling rate for cyropreservation of platelets.Methods: Platelet concentrate was cooled down with the new cryopreserving solution at various cooling rates(1,10,20℃/min) and was kept at -80℃ for one week．Then platelets were thawed to check platelet count，adrenaline induced aggregation，and expression of CD41,CD42b and CD62p; the results were compared with those of the non-programmed cooling group and fresh platelets.Results: The platelet counts of cryopreserved groups were lower than that of fresh platelet concentrate(P<0.05 or P<0.01).There were no differences in adrenaline induced aggregation between 10℃/min group,1℃/min group,and non-programmed cooling group; and the aggregation of the former 3 groups was lower than that of 1℃/min group(P<0.01) and higher than that of 20℃/min group (P<0.05).The CD42b expression in 10℃/min group was higher than in other groups (P<0.05) and lower than in fresh platelet concentrate.The CD62p expression in 1℃/min group,20℃/min group,none programme cooling group was higher than in fresh platelet concentrate(P<0.05).The CD62p expression in 10℃/min group was higher than other 3 groups (P<0.05) and lower than in fresh platelet concentrate,with no statistical significance.Conclusion: Cooling rate has great impact on the quality of cryopreservted platelets.Compared with 1℃/min and 20℃/min,10℃/min is the optimal cooling rate with the new cryopreservation solution in this study. |
| Key words: cryopreservation blood platelets platelet count CD41 antigen CD42b antigen CD62p antigen |
