| 摘要: |
| 目的:研究阻塞性黄疸大鼠离体胸主动脉收缩及舒张功能的变化规律,探讨高胆红素血症导致阻塞性黄疸大鼠离体胸主动脉收缩及舒张功能变化的可能机制。方法:60只雄性SD大鼠随机分为胆总管结扎(BDL)组和假手术(SO)组,每组30只,术后3、7、14 d,取大鼠离体胸主动脉环,分别测定大鼠胸主动脉对高浓度钾离子(K+)诱发的最大收缩幅度、梯度浓度去甲肾上腺素(NE)和去氧肾上腺素(PE)引起的收缩反应以及硝普钠(SNP)引起的舒张反应。另取40只雄性SD大鼠,随机分为SO组、BDL 3 d组、BDL 7 d组、BDL 14 d组,每组10只。取大鼠的胸主动脉组织,利用Western印迹方法检测大鼠胸主动脉肾上腺素α1D受体亚型(α1D-AR)蛋白表达情况。结果:高钾离子、NE、PE 在BDL大鼠离体胸主动脉引起的收缩反应弱于SO组,而硝普钠引起的舒张反应强于SO组；BDL组大鼠α1D-AR蛋白表达较SO组减弱,并且随阻塞时间的延长,减弱更加明显。结论:BDL大鼠离体胸主动脉的收缩与舒张功能在高胆红素血症时发生改变,α1D-AR蛋白表达下降可能是收缩功能减弱的原因之一。 |
| 关键词: 阻塞性黄疸 胸主动脉 血管收缩 血管舒张 |
| DOI：10.3724/SP.J.1008.2009.0162 |
| 投稿时间：2008-05-09修订日期：2008-11-06 |
| 基金项目:国家自然科学基金(30600584). |
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| Changes of vasoconstrictor and vasodilator functions of isolated thoracic aorta rings in rats with obstructive jaundice |
| WU Gang,XU Xue-wu,MIAO Xue-rong,YANG Li-qun,LI Quan,YU Wei-feng* |
| (Department of Anesthesiology,Eastern Hepatobiliary Hospital,Second Military Medical University,Shanghai 200438,China) |
| Abstract: |
| Objective:To investigate the changes of vasoconstrictor and vasodilator functions of isolated thoracic aorta in the rats with obstructive jaundice,so as to study the mechanism for the functional changes of the aorta in rats with obstructive jaundice.Methods: Sixty male SD rats were randomly divided into bile duct ligation (BDL) group and sham operation (SO) group.The thoracic aortas were isolated at 3,7 and 14 d after surgery and the vasoconstrictor responses induced by high potassium,norepinephrine (NE) and phenylephrine (PE) gradient and the vasodilator response to SNP were tested.Another 40 male SD rats were randomly divided into BDL three-day group,BDL seven-day group,BDL 14-day group and sham operation (SO) group.The expression of α1D -AR protein in the thoracic aorta was assessed by Western blotting.Results: The vasoconstrictor responses of thoracic aorta rings in high potassium,NE,PE,and BDL rats were weaker than those in the SO group.The vasodilator response induced by SNP in the BDL group was greater than that in the SO group.The expression of α1D -AR protein in the BDL group was weaker than that in the SO group,and the expression decreased with the prolongation of obstructive time.Conclusion: The systolic and diastolic functions of isolated thoracic aorta rings are changed in cholestatic rats; the weakened systolic function might be a result of decreased expression of α1D-AR albumen. |
| Key words: obstructive jaundice thoracic aorta vasoconstriction vasodilation |
