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骨肉瘤乳腺癌特异基因、Bcl-2及相关转录因子、雌激素受体mRNA的表达
雷鸣1,缪明永2,徐卫东1*
0
(1.第二军医大学长海医院骨科,上海 200433;2.第二军医大学基础部生物化学与分子生物学教研室,上海 200433)
摘要:
目的:探讨骨肉瘤乳腺癌特异基因1(breast carcinoma specific gene 1,BCSG1)、B细胞淋巴瘤/白血病-2(B cell lymphoma/leukemia-2,Bcl-2)、Bcl-2相关转录因子(Bcl-2-associated transcription factor,BTF)和雌激素受体(estrogen receptor,ER)基因表达水平及意义。方法:用实时荧光定量RT-PCR技术检测16例低(未)分化骨肉瘤标本及成骨细胞系hFOB 1.19细胞株BCSG1、BTF、Bcl-2、ERα、ERβ mRNA的表达,分析各指标间的相关性。结果:骨肉瘤组织中BCSG1、Bcl-2、ERα、ERβ的表达明显高于成骨细胞系hFOB 1.19细胞株(P<0.01),BTF表达两组无统计学差异;骨肉瘤组织ERα/ERβ显著高于成骨细胞系hFOB 1.19细胞株(P<0.01);肿瘤瘤体大小、患者性别对BCSG1、Bcl-2、BTF、ERα、ERβ的表达未见显著影响;骨肉瘤组织中BCSG1与ERα(r=0.696,P<0.01)、ERβ与Bcl-2(r=0.875,P<0.01)、ERα/ERβ与Bcl-2基因表达水平(r=-0.649,P<0.01)分别存在线性关系。结论:骨肉瘤组织中可能存在BCSG1增强ERα基因转录激活的作用,ERβ可能影响Bcl-2的表达。
关键词:  乳腺癌特异基因  Bcl-2  雌激素受体  转录因子  骨肉瘤
DOI:10.3724/SP.J.1008.2009.0416
投稿时间:2008-07-03修订日期:2009-03-02
基金项目:上海市基础研究重点项目(04JC14028).
mRNA expression of BCSG1, BTF, Bcl-2, ERα and ERβ in osteosarcoma and its significance
LEI Ming1, MIAO Ming-yong2, XU Wei-dong1*
(1.Department of Orthopedics, Changhai Hospital, Second Military Medical University, Shanghai 200433, China;2.Department of Biochemistry & Molecular Biology, College of Basic Medical Sciences, Second Military Medical University, Shanghai 200433)
Abstract:
Objective:To observe the mRNA expression of BCSG1(breast carcinoma specific gene,BCSG1), BTF(Bcl-2-associated transcription factor,BTF), Bcl-2(B cell lymphoma/leukemia-2,Bcl-2) and two subtypes of estrogen receptors in osteosarcoma and their implication. Methods: The expression of BCSG1, ERα, BTF, ERβ, and Bcl-2 mRNA were examined in 16 poorly differentiated osteosarcoma samples and a cell line hFOB 1.19 by quantitative RT-PCR. The relationship between their expression was analyzed. Results: The mRNA expression of BCSG1, Bcl-2 and two subtypes of estrogen receptors in the osteosarcoma tissues was significantly higher than that in the osteoblast cell line of hFOB 1.19; the expression of BTF was not significantly different. ERα/ERβ in the osteosarcoma tissues was significantly higher than that in the hFOB 1.19 cells (P<0.01). BCSG1, ERα, ERβ, Bcl-2 and BTF expression was not associated with the tumor size or patients gender. Correlation analysis showed there was a linear relationship between expression of BCSG1 and ERα,ERβ and Bcl-2, and ERα/ERβ ratio and Bcl-2. Conclusion: BCSG1 may enhance the activation of estrogen receptor-α gene transcription, and ERβ may influence the expression of Bcl-2 in osteosarcoma.
Key words:  breast carcinoma specific gene  Bcl-2  estrogen receptor  transcription factors  osteosarcoma