| 摘要: |
| 目的:采用高效液相色谱法测定人参Panax ginseng C.A.Mey.中8种主要皂苷类成分的含量。 方法:人参药材以甲醇超声30 min提取。采用Agilent Zorbax SB-C18 色谱柱(3.0 mm×100 mm,3.5 μm),流动相为乙腈(A)和水(B),梯度洗脱;A相含量随时间的变化为18%～20%(0～14 min),20%～29%(14～20 min),29%(20～25 min),29%～37%(25～34 min),37%～55%(34～40 min)；流速0.6 ml/min；检测波长203 nm；柱温25℃；进样量10 μl。 结果:8种人参皂苷Rb1、Rb2、Rb3、Rc、Rd、Re、Rf、Rg1在35 min内基线分离,线性良好(r=0.999 9)。方法学考察表明,日内日间精密度,最低检测限和定量限的范围均符合相关标准,加样回收率(n=3)分别为101.07%、98.72%、101.57%、101.71%、102.12%、99.58%、98.62%、100.12%。测定了3个厂家6个批次人参药材中8种人参皂苷的含量。结论:该方法快速简便,稳定可靠,可以用来对人参药材进行质量控制。 |
| 关键词: 人参 人参皂苷 高效液相色谱法 含量测定 |
| DOI：10.3724/SP.J.1008.2008.01507 |
| 投稿时间：2008-08-04修订日期：2008-09-27 |
| 基金项目: |
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| High performance liquid chromatography in rapid determination of eight ginsenosides in the root of Panax ginseng |
| ZHAO Liang1,L Lei1,JI Song-gang2,LI Jie-wei1*,Li Jie-wei |
| (1.Department of Pharmacy,Eastern Hepatobiliary Hospital,Second Military Medical University,Shanghai 200438,China*2.Department of Pharmacy,No.401 Hospital of PLA,Qingdao 266071;Department of Pharmacy,East Hepotobiliary Surgery Hospital,Second Military Medical University) |
| Abstract: |
| Objective:To determine the contents of eight ginsenosides in the root of Panax ginseng by high performance liquid chromatography (HPLC). Methods:Panax ginseng was extracted with methanol solution for 30 min.The HPLC condition was as follows-column:Agilent SB-C18(3.0 mm×100 mm,3.5 μm); mobile phase:A was ACN,B was H2O,with gradient elution;the gradient of A phase:18%-20%(0-14 min),20%-29%(14-20 min),29%(20-25 min),29%-37%(25-34 min),37%-55%(34-40 min); flow speed:0.6 ml/min; detection:203 nm; temperature of column:25℃;injection volume:10 μl. Results:Eight ginsenosides (Rb1,Rb2,Rb3,Rc,Rd,Re,Rf and Rg1) were separated at baseline within 35 min with good linearity (r=0.9999).The result of intra-day and inter-day precisions,limits of detection and quantitation were all within the normal range. The recovery rates(n=3) were 101.07%,98.72%,101.57%,101.71%,102.12%,99.58%,98.62%,and 100.12%,respectively. The contents of eight ginsenosides in 6 different batches of Panax ginseng from 3 sources were determined.Conclusion:The present method is rapid,simple,accurate and can be used to control the quality of Panax ginseng. |
| Key words: Panax ginseng ginsengosides high performance liquid chromatography determination |
