| 摘要: |
| 目的:从细胞及动物整体水平研究银耳多糖(tremella polysaccharides, TP)抗心肌细胞凋亡的作用。方法:采用醇提法提取精制TP,气相色谱法检测TP纯度。对乳鼠心肌细胞原代培养后随机分为正常对照组（A组）,凋亡对照组（B组）和TP预处理组（T组）,72 h后进行形态学观察和锥虫蓝摄取率测定。采用流式细胞术检测各组心肌细胞凋亡指数。在动物体内实验中,选择清洁级ICR纯系小鼠100只,按体质量随机分为5组：阴性对照组（N组）每天腹腔注射生理盐水,其余4组每天腹腔注射120 mg/kg D-半乳糖以建立小鼠衰老模型,同时阳性对照组（C组）每日以生理盐水灌胃,L、M、H组分别以每日100、200、400 mg/kg TP灌胃。8周后处死小鼠,获取心肌标本进行组织学研究和生化检测。结果:培养的各组乳鼠心肌细胞锥虫蓝摄取率：B>C>A（P<0.01）；心肌细胞凋亡指数：B>C>A（P<0.01）。动物实验中,与N组相比,阳性对照组（C组）及各TP干预组心肌均无明显病理学改变。TUNEL法检测心肌细胞凋亡指数显示：H |
| 关键词: 银耳多糖 细胞凋亡 心肌细胞 抗氧化 |
| DOI：10.3724/SP.J.1008.2009.0383 |
| 投稿时间：2008-11-07修订日期：2009-02-23 |
| 基金项目:国家自然科学基金(30672733). |
|
| Anti-apoptosis effect of tremella polysaccharides on cardiomyocytes |
| QU Dan1,CAI Dong-lian1*,ZHANG Hao2,QIAO Xiao-ping1,HE Fang1,WANG Ying1,LIN Ning1 |
| (1. Department of Nutrition, Changhai Hospital, Second Military Medical University, Shanghai 200433, China;2. Department of Cardiothoracic Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433) |
| Abstract: |
| Objective:To observe the anti-apoptosis effect of tremella polysaccharides (TP) on cardiomyocytes by in vitro and in vivo experiments. Methods: TP was extracted by dehydrated alcohol and the TP purity was measured by gas chromatography. The cultured cardiomyocytes of the neonatal rats were randomly assigned to normal group (Group A), apoptosis-induced group (Group B) and TP-pretreatment group (Group T). The morphology and uptake rate of trypan blue were studied after 72 h. The apoptosis index of cardiomyocyte was measured by flow cytometry. In the in vivo study, 100 ICR mice were randomly assigned to 5 groups after paired by body weight as following: Group N (negative control group, peritoneally injected by saline daily); Group C (positive control group, peritoneally injected by D-galactose and saline daily); Group L, M, and H (peritoneally injected by D-galactose and intragastric administered with TP at 100, 200, 400 mg/day, respectively). Mice were sacrificed for histology and biochemistry studies after 8 weeks. Results: Data of cultured cardiomyocytes were as follows: Uptake rate of trypan blue: B>C>A (P<0.01)；apoptosis index：B>C>A (P<0.01). In the animal studies there were no obvious difference in myocardium pathology between groups. The order of the apoptosis indices of myocardium was H |
| Key words: tremella polysaccharides apoptosis cardiomyocyte antioxidation |
