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从噬菌体展示肽库中筛选 PreS1抗原的结合肽
陈青青,郑慧敏,郭葆玉*
0
(第二军医大学药学院生化药学教研室,上海 200433)
摘要:
目的:从噬菌体随机七肽库中筛选能与PreS1抗原特异性结合的多肽。方法:利用噬菌体展示技术,以PreS1抗原为靶分子,从随机七肽库中进行生物亲和筛选,ELISA鉴定阳性克隆。结果:对肽库进行3 轮筛选后,通过ELISA和竞争抑制ELISA,获得了特异性的结合肽,测序结果显示噬菌体上的短肽有共同序列。结论:利用噬菌体展示技术成功筛选到了PreS1抗原的结合肽,为乙肝的治疗和诊断探索新的途径。
关键词:  PreS1抗原  噬菌体展示肽库  结合肽  乙型肝炎
DOI:10.3724/SP.J.1008.2009.0783
投稿时间:2008-11-10修订日期:2009-05-11
基金项目:
Selection of peptides bind to PreS1 antigen from a phage-displayed peptide library
CHEN Qing-qing,ZHENG Hui-min,GUO Bao-yu*
(Department of Biochemical Pharmacology,School of Pharmacy,Second Military Medical University,Shanghai 200433,China)
Abstract:
Objective:To screen for peptides that specifically bind to PreS1 antigen from a phage-displayed peptide library.Methods: The PreS1 antigen was used as the target molecule to screen the binding peptide from the Ph.D.-7 peptide library with phage display technique,and the positive clones were identified by ELISA.Results: After three rounds of biopanning,the binding peptides were screened from the peptide library by ELISA and competitive inhibiting ELISA.Sequencing result showed that the binding peptides had high affinity and specificity.Conclusion: A peptide binding PreS1 antigen has been successfully obtained by screening the phage display library,which paves a way for the diagnosis and treatment of hepatitis B infection.
Key words:  PreS1 antigen  phage display peptide library  binding peptide  hepatitis B