摘要: |
目的:观察早期肾透明细胞癌及癌旁0.5、1.0、2.0 cm组织上皮膜抗原(epithelial membrane antigen,EMA)、肾细胞癌标志物(renal cell carcinoma marker,RCC-Ma)的表达差异,初步探讨早期肾癌行肾部分切除术时的安全切除边距。方法:构建包含44例早期肾透明细胞癌及癌旁0.5、1.0、2.0 cm肾皮质的组织芯片,采用免疫组化染色分别观察癌及癌旁(距肿瘤边缘0.5、1.0、2.0 cm)组织EMA、RCC-Ma的表达差异。结果:EMA、RCC-Ma在癌及癌旁组织均为细胞膜和(或)胞质染色。癌组织EMA和RCC-Ma强阳性表达率明显高于癌旁组织各组(15.91%/18.18% vs 84.09%/79.55%、86.36%/77.27%、79.55%/75.00%,P<0.01);而癌旁组织各组(距肿瘤边缘0.5、1.0、2.0 cm)间差异无统计学意义(P>0.05)。结论:0.5 cm可能是早期肾透明细胞癌腹腔镜肾部分切除术安全的切除边距。 |
关键词: 上皮膜抗原 肾细胞癌标志物 肾肿瘤 组织芯片 肾切除术 |
DOI:10.3724/SP.J.1008.2009.0672 |
投稿时间:2008-11-21修订日期:2009-01-04 |
基金项目:上海市科委医学引导科技项目(074119618). |
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Determination of safe margin of laparoscopic partial nephrectomy for early renal carcinoma |
SHEN Yi-zhen1,ZHENG Jun-hua2*,YU Guan-zhen3,GAO Yi1,MIN Zhi-lian1 |
(1.Department of Urology,Changzheng Hospital,Second Military Medical University,Shanghai 200003,China;2.Department of Urology,The 10th People’s Hospital of Shanghai,Tongji University,Shanghai 200072;3.Department of Oncology,Changzheng Hospital,Second Military Medical University,Shanghai 200003) |
Abstract: |
Objective:To observe the differential expression of epithelial membrane antigen (EMA) and renal cell carcinoma marker (RCC-Ma) in early renal carcinoma and corresponding adjacent (0.5, 1.0, and 2.0 cm) tissues, so as to estimate the safe margin of laparoscopic partial nephrectomy for renal tumors.Methods:Tissue microarrys, including 44 tissues of clear cell renal cell carcinoma[A] and normal renal cortex tissues of different distances to the tumor:0.5 cm[B],1.0 cm[C] and 2.0 cm[D], were constructed.The expression of EMA and RCC-Ma was examined by immunohistochemical staining in the four groups. Results:Intensive membranous and(or) cytoplasmic staining of EMA and RCC-Ma was observed in the cancer and adjacent tissues. The positive rate in group A was significantly higher than those of the other three groups(A \[15.91%/18.18%\],B\[84.09%/79.55%\],C\[86.36%/77.27%\] and D\[79.55%/75.00%\], P<0.01).The positive rates of EMA and RCC-Ma expression were not significantly different between the group B, C, and D(P>0.05).Conclusion:Our data denote that, when laparoscopic partial nephrectomy is done for early renal carcinoma, at least 5 mm of normal parenchyma tissue beyond the pesudocapsule should be excised with the tumor. |
Key words: epithelial membrane antigen renal cell carcinoma marker kidney neoplasms tissue microarray nephrectomy |