| 摘要: |
| 目的:分离人参内生真菌，并探讨其代谢产物粗提物的体外抗真菌、抗肿瘤活性。方法：首次对5年生园参和15年生移山参内生真菌进行了分离，以稻瘟霉（Pyricularia oryzae P-2b）为筛选模型，采用微量稀释法筛选人参内生真菌体外抗病原真菌活性，MTT法检测其体外抗肿瘤活性。结果：从人参中共计分离得到内生真菌48株，其中16株（33.3%）能完全抑制稻瘟霉活性。16株具有活性的内生真菌中11株（22.9%，11/48）抗白念珠菌（Candida albicans）、新生隐球菌（Cryptococcus neoformans）、红色毛癣菌（Trichophyton rubrum）、薰烟曲霉菌（Aspergillus fumigatus）活性较好；5株（10.4%，5/48）抗胃癌细胞（MKN45）、肠癌细胞（LOVO）、肝癌细胞（HepG2）、白血病细胞（HL-60）活性较好。其中，以园参-25（Yuan-25）抗真菌活性最佳，其抗红色毛癣菌的MIC80为4 mg/L，与阳性对照氟康唑的抗菌活性相当；园参-27（Yuan-27）抗肿瘤活性最优，其抗肿瘤细胞的IC50均与阳性对照阿霉素较接近。结论：分离得到的人参内生真菌具有潜在的抗肿瘤、抗真菌活性，值得进一步研究和开发。 |
| 关键词: 人参 内生真菌 分离 生物活性 |
| DOI：10.3724/SP.J.1008.2009.0699 |
| 投稿时间：2008-12-19修订日期：2009-04-24 |
| 基金项目: |
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| Isolation of endophytic fungi from Panax ginseng and their antifungal and antitumor activities in vitro |
| XU Li-li,HAN Ting,LI Lin,QIN Lu-ping* |
| (Department of Pharmacognosy,School of Pharmacy,Second Military Medical University,Shanghai 200433,China) |
| Abstract: |
| Objective:To isolate endophytic fungi from Panax ginseng and study their antifungal and antitumor activities.Methods:Endophytic fungi were isolated from 5-year-old garden ginseng and 15-year-old transplanted ginseng.The antifungal active endophytes were screened with Pyricularia oryzae P-2b model using mircodilution method, and the activities of endophytes against pathogenic fungi were tested in vitro. The antitumor activities of the endophytes were examined by MTT method in vitro. Results:Sixteen (33.3%) of the 48 endophytic fungi fully suppressed the activity of P.oryzae P-2b; 11 (22.9%) colonies showed satisfactory antifungal activities against Candida albicans, Cryptococcus neoformans, Trichophyton rubrum, and Aspergillus fumigatus; and 5 (10.4%) colonies showed satisfactory antitumor activities against tumor cell lines MKN45, LOVO, HepG2, and HL-60. Among the bioactive colonies, Yuan-25 showed best antifungal activity, with its MIC80 aganinst Trichophyton rubrum being 4 mg/L, which was similar to that of fluconazole. Yuan-27 showed the best antitumor activity, with its IC50 similar to that of doxorubicin.Conclusion:Isolated endophytic fungi of Panax ginseng has antifungal and antitumor activities and is worth further exploring. |
| Key words: Panax ginseng endophytic fungi isolation bioactivity |
