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| 利用分叶顺次肝切除术建立小鼠肝脏大部切除后再生模型 |
| 汤朝晖1,周露婷2,谢志芳3,胡以平3,杨甲梅1*,章卫平2*,吴孟超1 |
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| (1.第二军医大学东方肝胆外科医院,上海 200438;2.第二军医大学基础部病理生理学教研室,上海 200433;3.第二军医大学基础部细胞生物学教研室,上海 200433) |
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| 摘要: |
| 目的:利用肝部分切除术,建立可准确量化和易于操控的小鼠肝脏再生模型,为研究肝脏再生的细胞和分子生物学机制及其病理学意义提供技术平台。方法:正常成年C57BL/6小鼠经心脏灌注固定后,逐叶分离肝脏、称重,计算各肝叶所占肝重的百分比；麻醉状态下,分叶顺次无菌切除小鼠肝脏的左外叶、左中叶和右中叶,建立肝脏大部切除的再生模型；利用RT-PCR方法动态检测术后肝脏甲胎蛋白基因的激活表达特征。结果: 肝左外叶、左中叶和右中叶合计约占小鼠肝脏总重量的68%,分叶顺次切除上述3个肝叶,术后动物恢复和存活状态良好,RT-PCR结果证实甲胎蛋白基因在小鼠肝脏再生中的激活表达。结论:利用分叶顺次肝切除术,成功建立了小鼠的肝大部切除后再生模型,该方法可准确量化肝脏切除的程度,具有可控性强、简便易行和成功率高等优点,为小鼠肝再生的研究奠定了基础。 |
| 关键词: 肝解剖 肝部分切除术 肝再生 |
| DOI：10.3724/SP.J.1008.2009.0524 |
| 投稿时间：2008-12-29修订日期：2009-04-23 |
| 基金项目:国家自然科学基金(90608026,30772478). |
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| Establishment of mouse liver regeneration model by consecutive partial hepatectomy |
| TANG Zhao-hui1,ZHOU Lu-ting2,XIE Zhi-fang3,HU Yi-ping3,YANG Jia-mei1*,ZHANG Wei-ping2*,WU Meng-chao1 |
| (1.Eastern Hepatobiliary Hospital,Second Military Medical University,Shanghai 200438;2.Department of Pathophysiology,College of Basic Medical Sciences,Second Military Medical University,Shanghai 200433;3.Department of Cell Biology,College of Basic Medical Sciences,Second Military Medical University,Shanghai 200433) |
| Abstract: |
| Objective:To establish a quantifiable and easily manipulable mouse model of liver regeneration by partial hepatectomy,so as to provide a basis for investigating the underlying cellular and molecular mechanisms and pathophysiological significance of liver regeneration.Methods:Normal adult C57BL/6 mice were fixed by cardiac perfusion,the liver lobes were dissected and weighed,and the proportions of each lobe were calculated.Under anesthetic and sterile condition,partial hepatectomy was performed by removing the left lateral,left middle,and right middle liver lobes in turn to establish the regeneration model.The activation of alpha-fetoprotein (AFP) gene in the regenerating liver was dynamically monitored by real-time PCR analysis.Results:The left lateral,left middle,and right middle liver lobes together accounted for approximately 70% of the total liver weight.The animals survived and lived well after removal of the three lobes in turn.RT-PCR showed that AFP expression was activated in the regenerating livers.Conclusion:We have successfully established the mouse model of liver regeneration by consecutive partial hepatectomy.This method can quantify the heptectomy and is easy to perform,which lays a foundation for studying liver regeneration in mice. |
| Key words: liver anatomy partial hepatectomy liver regeneration |
