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原发性胆汁性肝硬化患者血清中Th17相关细胞因子的表达
周运恒,荣光华,熊怡淞,朱烨,耿红莲,杨再兴,仲人前*
0
(第二军医大学长征医院实验诊断科,全军医学免疫诊断中心,全军临床免疫重点实验室,上海 200003)
摘要:
目的:观察原发性胆汁性肝硬化(primary biliary cirrhosis,PBC)患者血清中Th17分化相关细胞因子(IL-1β、IL-6、IL-17、IL-23、TGF-β)的变化,探讨Th17细胞在PBC发病过程中的作用。方法:采用酶联免疫吸附试验(ELISA)方法比较PBC患者(n=78)、乙型肝炎后肝硬化患者(PHC,n=30),健康对照(HC,n=60)血清中Th17分化相关细胞因子含量。结果:与HC组相比,PBC患者血清IL-1β、IL-6、IL-17A和IL-23水平显著升高(P<0.05),而TGF-β水平差异无统计学意义;与PHC组相比,PBC患者血清IL-1β、IL-6和TGF-β水平显著降低,而IL-17和IL-23水平显著升高(P<0.05)。结论:PBC患者处于慢性炎症状态,Th17细胞可能在PBC的发病和发展过程中发挥着重要的作用。
关键词:  原发性胆汁性肝硬化  Th17细胞  细胞因子
DOI:10.3724/SP.J.1008.2009.0703
投稿时间:2009-01-06修订日期:2009-04-20
基金项目:国家高科技研究发展计划(“863”计划,2006AA02Z496),上海市科学技术委员会优秀学科带头人基金(07XD14013).
Serum profile of Th17-related cytokines in patients with primary biliary cirrhosis
ZHOU Yun-heng,RONG Guang-hua,XIONG Yi-song,ZHU Ye,GENG Hong-lian,YANG Zai-xing,ZHONG Ren-qian*
(Department of Laboratory Medicine,Key Laboratory and Clinical Immunology Center of PLA,Changzheng Hospital,Second Military Medical University,Shanghai 200003,China)
Abstract:
Objective:To observe the serum profile of Th17-related cytokines(IL-1β,IL-6,IL-17A,IL-23, and TGF-β)in patients with primary biliary cirrhosis,so as to study the role of Th17 cells in the pathogenesis of primary biliary cirrhosis (PBC).Methods:Seventy-eight patients with PBC,30 patients with HBV-related posthepatitic cirrhosis(PHC),and 60 healthy controls (HC) were enrolled in this study.The serum levels of above five Th17-related cytokines were determined by enzyme-linked immunosorbent assay (ELISA).Results:The levels of IL-1β,IL-6,IL-17A, and IL-23 in PBC group were significantly higher than those in HC group (P<0.05); the levels of TGF-β were comparable between the PBC group and HC group.The levels of IL-1β,IL-6, and TGF-β were significantly lower and IL-17A,IL-23 were significantly higher in the PBC group compared with those in the PHC group (P<0.05).Conclusion:Our findings indicate that PBC patients are in a status of chronic inflammation.Th17 subset may play an important role in the development and progression of PBC.
Key words:  primary biliary cirrhosis  Th17 cells  cytokines