| 摘要: |
| 目的:探讨哺乳动物雷帕霉素靶蛋白（mammalian target of rapamycin,mTOR）及其活化形式磷酸化mTOR（phosphorylated mTOR, p-mTOR）在人大肠癌发生发展中的作用及临床意义。方法:应用组织芯片和免疫组织化学技术检测185例大肠癌组织及癌旁组织中mTOR及p-mTOR的表达，并分析其与大肠癌临床病理学特征如年龄、性别、肿瘤浸润深度（T分期）、淋巴转移、TNM分期和分化程度之间的关系。结果:在大肠癌旁组织中mTOR呈现弥散表达，p-mTOR基本不表达或散在表达，两者在大肠癌组织中的表达强度明显高于癌旁组织。mTOR 和p-mTOR在大肠癌中的过表达率分别为45.9%和42.2%。两者均与性别、年龄无显著相关。mTOR的过表达与大肠癌的分化程度相关（P<0.05），而与浸润深度（T分期）、TNM分期、淋巴转移无显著相关（P>0.05）。p-mTOR的过表达与大肠癌的浸润深度（T分期）、淋巴转移和TNM分期显著相关（P<0.05），而与分化程度无显著相关（P>0.05）。结论:p-mTOR的过表达与人大肠癌的恶性表型密切相关，mTOR的磷酸化可能参与调控了大肠癌的发生发展。 |
| 关键词: 组织芯片 雷帕霉素 mTOR p-mTOR 结直肠肿瘤 |
| DOI：10.3724/SP.J.1008.2009.0517 |
| 投稿时间：2009-01-06修订日期：2009-02-15 |
| 基金项目: |
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| Expression of phosphorylated mammalian target of rapamycin in colorectal cancer and its significance |
| XIANG Hong-gang,HU Zhi-qian,WANG Wei-jun,XU Jian,ZHANG Jian,XU Chao,WANG Qiang* |
| (Department of General Surgery,Changzheng Hospital,Second Military Medical University,Shanghai 200003,China) |
| Abstract: |
| Objective:To explore the roles of mammalian target of rapamycin (mTOR) and the activated mTOR (phosphorylated mTOR,p-mTOR) in the development and progression of colorectal cancer,and to discuss the clinical significance.Methods:The expression of mTOR and p-mTOR in 185 colorectal cancer specimens and the corresponding adjacent tissues were evaluated by tissue microarray and immunohistochemistry,and the relationship between the expression and the age,sex,invasion depth(T stage),lymph metastasis,TNM stage and differentiation degree was analyzed.Results:Diffused expression of mTOR and hardly any expression of p-mTOR were found in the adjacent tissues.The expression of mTOR and p-mTOR was obviously stronger in the colorectal cancer tissues compared with that in the adjacent tissues.The over-expression rates of mTOR and p-mTOR in colorectal cancer were 45.9% and 42.2%,respectively.There was no significant correlation of mTOR and p-mTOR over-expression with age,sex(P>0.05); the over-expression of mTOR was correlated with the differentiation degree (P<0.05),but not with the invasion depth (T stage),TNM stage,or lymph metastasis (P>0.05).The correlation of p-mTOR over-expression with the invasion depth (T stage),lymph metastasis and TNM stage was significant (P<0.05),but that with the differentiation degree was not significant (P>0.05).Conclusion:Over-expression of p-mTOR is closely associated with the malignant phenotype of colorectal cancer.It is also indicated that p-mTOR may be involved in the development and progression of colorectal cancer. |
| Key words: tissue microarray rapamycin mTOR p-mTOR colorectal neoplasms |
