| 摘要: |
| 目的:研究雌激素对缺血性脑损害的神经保护作用是否与抗神经元凋亡有关。方法:30只雌性成年SD大鼠,随机分为假手术组(sham),手术对照组(OVX),替勃龙组\[0.25 mg/(kg·d)\];采用大鼠大脑中动脉闭塞制成局灶性脑缺血模型。在缺血2 h、再灌注24 h后立即断头取脑,应用2,3,5-三苯基氯化四唑(TTC)染色观察脑梗死体积,TUNEL法检测凋亡细胞,链酶菌抗生物素蛋白过氧化物酶(SP)法检测抗凋亡基因Bcl-2、细胞凋亡诱导因子Bax和半胱氨酰天冬氨酸特异性蛋白酶caspase-3的表达。结果:替勃龙用药组较手术对照组脑梗死体积显著缩小(P<0.05),TUNEL阳性细胞数减少(P<0.01),Bcl-2阳性细胞增多(P<0.01),Bax及caspase-3阳性细胞减少(P<0.01)。结论:替勃龙可抑制去卵巢大鼠局灶性脑缺血再灌注时神经细胞凋亡,具有神经保护作用。 |
| 关键词: 替勃龙 脑缺血 再灌注损伤 细胞凋亡 |
| DOI：10.3724/SP.J.1008.2009.0790 |
| 投稿时间：2009-01-12修订日期：2009-05-11 |
| 基金项目:河北省科技厅科技基金(03276196D-52). |
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| Effects of tibolone on apoptosis of neurons after cerebral ischemia-reperfusion injury in rats |
| TIAN Wen-yan1 ,ZHANG Hong-yi 2 , SU Li-kai1 ,SHEN Wen-zeng2 |
| (1.Department of Neurology,Affiliated Hospital of Hebei University,Baoding 071000,China;2.Experimental Center,College of Basic Medical Sciences,Hebei University,Baoding 071000) |
| Abstract: |
| Objective:To study whether the neuroprotective effect of tibolone during cerebral ischemia injury is associated with its anti-apoptosis effect.Methods: A total of 30 healthy adult female Sprague Dawley rats were evenly randomized into sham-operated group,ovariectomized group,and tibolone group（0.25 mg/\[kg·d\]).Brain ischemia model was induced by left middle cerebral artery occlusion(MCAO) for 2 hours followed by 24 hours reperfusion.All animals were killed and the cerebral infarction volumes were determined by 2,3,5-triphenyltetrazlium chloride (TTC) staining and image analysis.Apoptotic cells were labeled by Terminal deoxynucleotidyl Transferase-mediated dUTP nick end labeling (TUNEL) .The expression of Bcl-2,Bax,and caspase-3 was analyzed by immunohistochemical SP method. Results: Compared with the sham-operated group(P<0.05),cerebral infarction volume was significantly reduced,TUNEL positive cells was significantly decreased(P<0.01),Bcl-2 positive cells were significantly increased,and Bax(P<0.01),caspase-3 postive cells were significantly reduced compared with tibolone treated groups (P<0.01).Conclusion: Tibolone can inhibit the neuron apoptosis following cerebral ischemia injury in OVX rats,displaying a neuroprotective effect. |
| Key words: tibolone cerebrovascular disorders reperfusion injuries apoptosis |
