| 摘要: |
| 目的:探讨原花青素(PC)预处理对体外人淋巴母细胞AHH-1和肠腺上皮细胞HIEC电离辐射损伤的防护效果及可能的作用机制。方法:采用CCK-8法检测原花青素预处理对γ射线照射后AHH-1、HIEC细胞存活率的影响,Annexin-Ⅴ/PI染色和流式细胞术分析细胞凋亡及细胞周期的变化,蛋白质印迹法检测细胞Bcl-2蛋白表达变化。结果:PC预处理的细胞辐照后存活率明显升高（P<0.01）,4 Gy照射用药组 AHH-1细胞凋亡率（11.78%）显著低于单纯照射组（26.38%,P<0.01）,8 Gy照射用药组HIEC细胞凋亡率（5.32%）亦显著低于单纯照射组（12.45%,P<0.01）。PC预处理组AHH-1、HIEC细胞受照射后其Bcl-2蛋白表达下降受到抑制。 结论:PC预处理对细胞的辐射损伤具有较好的防护效果,可能与其促进受损细胞Bcl-2蛋白表达有关。 |
| 关键词: 原花青素类 辐射损伤 淋巴母细胞 肠腺上皮细胞 Bcl-2蛋白 |
| DOI：10.3724/SP.J.1008.2009.0641 |
| 投稿时间：2009-01-20修订日期：2009-05-20 |
| 基金项目:国家自然科学基金（30470415,30800284）. |
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| Protective effects of proanthocyanidin on radiation-induced injury of human-derived cells |
| GAO Fu,LI Bai-long,SUN Ding,HUANG Yue-cheng,NI Jin,CUI Jian-guo,ZHAO Fang,CAI Jian-ming* |
| (Department of Radiation Medicine,Faculty of Navy Medicine,Second Military Medical University,Shanghai 200433,China) |
| Abstract: |
| Objective:To investigate the protective effects of proanthocyanidin on radiation-induced injury of AHH-1 and HIEC cells,and to explore the possible molecular mechanism.Methods: CCK-8 assay was applied to examine the survival of AHH-1,HIEC cells after γ-ray irradiation with or without proanthocyanidin pretreatment.Annexin-Ⅴ/PI staining and flow cytometry analysis were used to evaluate the apoptosis and cell cycle of AHH-1 and HIEC cells.The expression of Bcl-2 protein was analyzed by Western blotting assay.Results: Pretreatment with proanthocyanidin significantly increased the cell survival rate after radiation(P<0.01).The apoptosis rates of cells in the proanthocyanidin+radiation group were (11.78% at 4 Gy\[AHH-1\] and 5.32% at 8 Gy\[HIEC\],respectively) greatly lower than those of the single radiation group (26.38% at 4 Gy\[AHH-1\] and 12.45% at 8 Gy\[HIEC\],respectively,P<0.01).Moreover,the inhibition of Bcl-2 protein expression in AHH-1,HIEC cells was attenuated in the proanthocyanidin+radiation group.Conclusion: Proanthocyanidin has satisfactory protective effect on radiation-induced cellular injury; the mechanism may be related to the attenuation of Bcl-2 protein inhibition. |
| Key words: proanthocyanidins radiation iujuries lymphoblast cell HIEC cell Bcl-2 protein |
