| 摘要: |
| 目的观察蒺藜甾体皂苷类化合物TTS-12对白念珠菌生物被膜形成的影响,探讨其可能的作用机制。方法采用激光共聚焦显微镜观察TTS-12对白念珠菌生物被膜生长形态的影响；采用XTT法考察不同浓度TTS-12对白念珠菌生物被膜形成的影响；应用光密度值测定及实时定量RT-PCR(real- time RT-PCR)法考察不同浓度TTS-12对白念珠菌细胞表面疏水性(cell surface hydrophobicity,CSH)及相关基因表达的影响。结果与未加TTS-12的正常对照细胞相比,经TTS-12处理的白念珠菌生物被膜结构疏松,TTS-12可剂量依赖性地抑制白念珠菌生物被膜的形成,其生长动力学及细胞表面疏水性值下降,CSH1基因表达水平降低(P<0.01)。结论TTS-12可能通过降低白念珠菌CSH1基因表达来抑制白念珠菌生物被膜的形成。 |
| 关键词: 白念珠菌 生物被膜 蒺藜 TTS-12 细胞表面疏水性 |
| DOI：10.3724/SP.J.1008.2010.0132 |
| 投稿时间：2009-02-04修订日期：2009-12-18 |
| 基金项目: |
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| Inhibitory effects of Tribulus terrestris L. steroid saponin TTS-12 on formation of Candida albicans biofilm |
| LU Wen-quan1, CAO Ying-ying2, CHEN Hai-sheng3, JIANG Yuan-ying2* |
| (1. Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China;2. Department of New Drug Research Center, School of Pharmacy, Second Military Medical University, Shanghai 200433, China;3. Department of Natural Product Chemistry, School of Pharmacy, Second Military Medical University, Shanghai 200433, China) |
| Abstract: |
| ObjectiveTo investigate the influence of TTS-12, a steroid saponin from Tribulus terrestris L., on the formation of Candida albicans biofilm, and to discuss the possible mechanism. MethodsThe inhibition of C.albicans biofilm formation by TTS -12 was observed by confocal scanning laser microscopy; XTT method was used to investigate the influence of different concentrations of TTS-12 on C.albicans biofilm formation. The water-hydrocarbon two-phase assay was used to measure the cell surface hydrophobicity of C.albicans treated with different concentrations of TTS-12. The expression of CSH1 mRNA was measured by real-time RT-PCR. ResultsCompared with control group, TTS-12 treatment resulted in loose C.albicans biofilm,and it dose-dependently inhibited C.albicans biofilm formation. The cell surface hydrophobicity in the TTS-12-treated groups was lower than that in the control group; consistent with this, TTS-12-treated cells also expressed significantly lower levels of CSH1 mRNA than cells in control group (P<0.01). ConclusionTTS-12 may inhibit the formation of C.albicans biofilm through inhibiting CSH1 gene expression. |
| Key words: Candida albicans biofilm Tribulus terrestris TTS-12 cell surface hydrophobicity |
