| 摘要: |
| 目的建立幽门螺杆菌（Helicobacter pylori,Hp）感染的兔颈动脉粥样硬化模型,为进一步探讨Hp与颈动脉粥样硬化的相关研究奠定基础。方法18只新西兰兔高脂喂养第6周时,随机抽取6只处死,另外12只兔随机平均分为对照组和实验组。实验组经兔耳缘静脉注射Hp标准菌株Sydney Strain 1（SS1）0.5 ml(4×108 CFU),每间隔24 h 注射1次,连续3次。对照组以等量生理盐水同样处理。实验第8周时,将所有实验动物处死。分别于实验前（第0周）、实验第6和第8周检测血脂水平；同期行颈动脉彩色多普勒超声检测颈动脉内中膜厚度（intima-media thickness,IMT）,观察斑块形成情况。以空气栓塞法处死动物后,留取颈动脉,肉眼观察血管形态、有无斑块以及斑块形态,10%甲醛固定,石蜡包埋、切片，H-E染色后光镜下观察血管病变并测量血管内膜厚度。结果所有动物均存活。高脂喂养第6周时成功建立新西兰兔高脂血症模型。实验第8周时,同对照组相比,实验组兔血脂水平、颈动脉IMT、血管内膜厚度均明显增加（P<0.05），颈动脉粥样硬化改变更为明显。结论在高脂血症新西兰兔基础上,经兔耳缘静脉注射Hp可成功建立颈动脉粥样硬化模型。 |
| 关键词: 幽门螺杆菌 高脂血症 内中膜厚度 颈动脉粥样硬化 动物模型 |
| DOI：10.3724/SP.J.1008.2010.037 |
| 投稿时间：2009-02-06修订日期：2009-03-26 |
| 基金项目:上海市浦江人才计划（08PJ1400300）,教育部留学回国人员科研启动基金（2008890）,上海市科委基础研究重点项目(09JC1400200). |
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| Establishment of a rabbit carotid atherosclerosis model by injection of Helicobacter pylori |
| TAO Zhen1,QU Le-feng2*,DING Su-ju1,JIA Qing-shuai3,LIAO Jian-sheng1,ZHAO Bao-zhen3 |
| (1.Department of Neurology,Changhai Hospital,Second Military Medical University,Shanghai 200433,China;2.Department of Vascular Surgery,Changhai Hospital,Second Military Medical University,Shanghai 200433,China;3.Department of Ultrasonography,Changhai Hospital,Second Military Medical University,Shanghai 200433,China) |
| Abstract: |
| ObjectiveTo establish a carotid arteriosclerosis model with New Zealand white rabbit by intravenous infusion of Helicobacter pylori(Hp),so as to lay a foundation for further investigating the relationship between Hp and carotid arteriosclerosis.MethodsEighteen New Zealand white rabbits were fed with high fat diet for six weeks;six of them were randomly chosen and sacrificed; the other twelve were evenly randomized into control group and experimental group.Animals in the experimental group were injected with 0.5 ml Sydney Strain 1(4×108 CFU) into the ear vein once a day for three days,and animals in the control group received normal saline in the same manner.All the animals were sacrificed on the eighth week.The blood lipid,carotid intima-media thickness (IMT),and plaque formation were observed before and 6,8 weeks after the intervention.The animals were sacrificed by air embolism and the carotid specimens were collected.The morphology of the blood vessels and the presence of plaque were observed with naked eye.H-E staining was used to observe the blood vessel diseases and intima thickness.ResultsAll the animals survived,and hyperlipidemia rabbit models were successfully established after 6-week feeding with high fat diet.The blood lipid level,carotid IMT,and blood vessel intima thickness were significantly increased in the experimental group compared with those in the control group at the eighth week（P<0.05）.More prominent atherosclerosis was noted in the experimental group compared with the control group on the eighth week.ConclusionCarotid arteriosclerosis model can be successfully established by intravenous injection of Hp in rabbits with hyperlipidemia. |
| Key words: Helicobacter pylori hyperlipidemias intima-media thickness carotid arteriosclerosis animal model |
