| 摘要: |
| 【摘要】：目的 建立检测大鼠小肠组织NF-κB及IκB-αmRNA表达水平的SYBR Green 实时定量PCR实验技术，观察海水浸泡伤大鼠小肠组织NF-κB及IκB-αmRNA表达水平的动态变化。方法 wistar大鼠63只，随机分为3组：空白对照组(n=7)，创伤组（分为12h、24h、48h、72h四组，每组n=7），海水浸泡+创伤组（分为12h、24h、48h、72h四组，每组n=7）。创伤组采用腹部开放伤模型，海水浸泡+创伤组在腹部开放伤的基础上海水浸泡1h。采用real-time PCR方法测定三组小肠粘膜组织NF-κB及IκB-αmRNA表达量并进行统计分析和组间比较。 结果 结果表明，与腹部开放创伤组相比，海水浸泡伤组大鼠小肠组织NF-κB mRNA在72h后出现显著表达水平上调（P<0.01），腹部开放创伤组和海水浸泡伤组大鼠小肠组织IκB-αmRNA在48h后都出现显著表达水平上调，但海水浸泡伤组在72h IκB-α mRNA表达上调更为显著（P<0.01）。结论 在海水浸泡伤的后期（＞72h），NF-κB mRNA表达明显上调，从而促进NF-κB蛋白水平的合成，从而进一步放大NF-κB信号转导途径，诱导炎症反应。同时， IκB-αmRNA表达水平的也明显上调促使IκB-α蛋白表达增加，以尽量下调细胞核中NF-κb的活性。 |
| 关键词: 腹部开放伤 海水浸泡 Toll样受体4 NF-κB I-κB |
| DOI：10.3724/SP.J.1008.2011.0688 |
| 投稿时间：2011-01-10修订日期：2011-05-12 |
| 基金项目:中国人民解放军总后勤部医药卫生科研计划课题(06D004). |
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| Real-time PCR in analysis of TLR4, NF-κB and IκB gene expression in small intestine of rats with open abdominal injury after seawater immersion |
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| Abstract: |
| 【ABSTRACT】： Objective : To establish a SYBR Green quantitative real-time PCR method to monitor the dynamics for NF-κB mRNA and IκB mRNA of opened abdominal injury with seawater immersion in rats. Methods: Wistar rats were randomly divided into three groups: the control group(n=7), the opened abdominal injury groups(n=28, all rats were randomly divided into four groups separately , which groups were 12h, ,24h, 48h ,and 72h after wounded) and opened abdominal injury with seawater immersion groups(n=28,a all rats were randomly divided into four groups separately , which groups were 12h, ,24h, 48h ,and 72h after wounded).The dynamics for the express of NF-κB mRNA and IκB mRNA of small intestine tissues was detected in every groups by real-time PCR method.. Results: NF-κB mRNA level was rise obviously after 72h in the opened abdominal injury with seawater immersion groups than other groups in same time point（P＜0.01）.The expression of IκB mRNA level was rise obviously after 48h in the opened abdominal injury with seawater immersion groups compared with the opened abdominal injury groups after 48h（P＜0.01）. Conclusions: The NF-κB signal pathways was activated and magnified in latter periods of the opened abdominal injury with seawater immersion（＞72h）, Which led to the development of continued inflammatory course ultimately. |
| Key words: opened abdominal injury seawater immersion real-time PCR NF-κB IκB mRNA |
