| 摘要: |
| 目的:采用大鼠腔前卵泡体外培养方法研究纳米级二氧化钛(TiO2)对大鼠卵泡发育及卵母细胞成熟的影响,揭示纳米TiO2有无雌性生殖毒性,为其安全性评价提供依据。方法:机械性分离大鼠腔前卵泡,单个卵泡在96孔板中连续培养。将卵泡随机分为5组:3个25 nm TiO2(12.5、25和50 μg/ml)组、阴性对照组、微米级TiO2对照组。培养第10天诱导排卵,观察卵泡发育和卵母细胞成熟情况。结果: 随着纳米级TiO2剂量增加，卵泡存活率、有腔形成率及卵丘-卵母细胞复合体(COCs)排出率呈下降趋势(P<0.05)；与阴性对照组相比,微米级TiO2组对卵泡发育与卵母细胞成熟无明显影响；25 μg/ml以上剂量纳米级TiO2组卵泡形态学明显异常,卵泡存活率及有腔形成率明显下降；50 μg/ml纳米级TiO2可使处于成熟阶段的卵母细胞数减少。结论:25 μg/ml剂量以上25 nm TiO2可以抑制体外大鼠腔前卵泡的生长发育与卵母细胞成熟,而微米级TiO2在相同剂量下对卵泡的生长发育和卵母细胞的成熟无明显影响。提示纳米级TiO2有不同于微米级TiO2的毒作用性质,可能有雌性生殖毒性,应引起关注。 |
| 关键词: 卵泡 卵母细胞 纳米级二氧化钛 毒性试验 |
| DOI：10.3724/SP.J.1008.2009.0869 |
| 投稿时间：2009-03-02修订日期：2009-06-01 |
| 基金项目:国家自然科学基金(30571587),国家重大科学计划子课题(2006CB932505),上海市科委纳米专项基金(0752nm020). |
|
| Effects of titanium dioxide nanoparticles on development and maturation of rat preantral follicle in vitro |
| HOU Juan,WAN Xu-ying,WANG Fei,XU Gui-feng,LIU Zhen,ZHANG Tian-bao* |
| (Department of Health Toxicology,College of Basic Medical Sciences,Second Military Medical University,Shanghai 200433,China) |
| Abstract: |
| Objective:To study the influence of titanium dioxide nanoparticle(TiO2) on follicle development and oocyte maturation of rats using in vitro rat preantral follicle culture system,so as to know whether TiO2 can cause female reproductive toxicity and provide evidences for evaluating its safety.Methods: Rat ovaries were mechanically dissected to obtain preantral follicles and the individual preantral follicle was cultured in 96-well plates.The study was divided into 5 groups: three 25 nm TiO2 groups (12.5 μg/ml,25 μg/ml and 50 μg/ml),one negative control group (treated with culture medium),and one micron TiO2 control group.Ten days after culture,the ovulation was induced and the follicle development and oocyte maturation were observed.Results:The survival rate of follicles,formation rate of antral follicles and release rate of cumulus-oocyte cell complexes (COCs) all decreased with the increase of 25 nm TiO2(P<0.05).Compared with the negative control group,micron TiO2 group had no obvious changes in the follicle development and oocyte maturation.Compared with the two control groups,25 nm TiO2 caused obvious morphological changes of follicles and obvious decrease in follicle survival and formation rate of antral follicles.50 μg/ml 25 nm TiO2 reduced the number of mature oocytes.Conclusion: 25 nm TiO2 at 25 μg/ml or above can inhibit rat follicle development and oocyte maturation in vitro,while the micron TiO2 at the same dose has no obvious influence on follicle development and oocyte maturation,suggesting different toxic properties between nmTiO2 and micron TiO2.Titanium dioxide nanoparticle may have female reproductive toxicity and should be given more attention. |
| Key words: ovarian follicle oocytes titanium dioxide nanoparticles toxicity test |
