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| PIAS2蛋白在不同病理分型的无精子症患者睾丸组织中的表达 |
| 冼志勇1,李飞1,邹亚光2,邓永键3,丁彦青3,毛向明1* |
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| (1.南方医科大学南方医院泌尿外科,广州 510515;2.南方医科大学南方医院口腔科,广州 510515;3.南方医科大学南方医院病理科,广州 510515) |
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| 摘要: |
| 目的:探讨PIAS2在不同生精功能状态的人睾丸组织中的表达情况。方法:对80例无精子症患者进行睾丸组织病理检查诊断,根据病理形态的不同分为：生精功能正常或基本正常(n=40)、精子发生低下(n=20)、唯支持细胞综合征(n=20)等。选择上述各型结构完整的睾丸组织,分别应用免疫组化法(SP)对PIAS2在不同生精功能状态的睾丸组织进行研究。结果:PIAS2在各级生精细胞多为强阳性表达,呈深棕黄色；PIAS2在间质细胞及支持细胞表达弱阳性表达或不表达。结论:PIAS2染色强弱与睾丸生精功能的强弱有一定关系,是一个潜在的判断睾丸生精功能的指标。 |
| 关键词: 男性不育 无精子症 PIAS2；睾丸；活组织检查；免疫组织化学染色 |
| DOI：10.3724/SP.J.1008.2009.0780 |
| 投稿时间：2009-03-15修订日期：2009-06-25 |
| 基金项目:国家自然科学基金(30772167)；广州市科技计划项目(2008J1-C131). |
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| Expression of PIAS2 in testis tissues of patients with different pathological types of azoospermia |
| XIAN Zhi-yong1,LI Fei1,ZOU Ya-guang2,DENG Yong-jian3,DING Yan-qing3,MAO Xiang-ming1* |
| (1.Department of Urinary Surgery,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China;2.Department of Stomatology,Nanfang Hospital,Southern Medical University,Guangzhou 510515 ;3.Department of Pathology,Nanfang Hospital,Southern Medical University,Guangzhou 510515) |
| Abstract: |
| Objective:To investigate the expression of PIAS2 in the testis tissues of patients with different spermatogenic functions.Methods:Eighty testicular biopsy samples from patients with azoospermia were subjected to pathological diagnosis and were divided into the following groups according to the pathological findings:normal or basically normal spermatogenesis group (n=40),hypo-spermatogenesis group (HS,n=20),and sertoli cell-only syndrome group (SCOS,n=20).Immunohistochemical method (SP) was used to examine the expression of PIAS2 in the testis tissues with different spermatogenic functions.Results:PIAS2 was strongly positive (dark brown) in the spermatogenic cells of different functions.It was weakly positive or absent in the Leydig cells and Sertoli cells.Conclusion:The status of PIAS2 in the testicular tissues is associated with the spermatogenic functions,and it may serve as a potential parameter for predicting of spermatogenic function. |
| Key words: male infertility azoospermia PIAS2 testis biopsy immunohistochemistry |
