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| 大鼠癫持续状态后脑内PPARγ的表达 |
| 于辛1*,邵晓光2,孙宏3,崔连奇1,黄远桂4 |
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| (1.解放军401医院神经内科,青岛266071;2.解放军401医院北院区内二科,青岛266100;3.西安交通大学第二附属医院神经内科,西安710004;4.第四军医大学西京医院神经内科,西安710032) |
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| 摘要: |
| 目的观察PPARγ在癫持续状态后大鼠脑内的表达。方法将雄性SD大鼠随机分为对照组和癫组(n=10)，制备锂-匹罗卡品癫模型，采用免疫组化及蛋白质印迹分析方法，观察对照组和造模成功的癫大鼠脑内PPARγ的表达分布和变化情况。采用免疫组化及蛋白质印迹分析方法，观察锂-匹罗卡品模型大鼠脑内PPARγ的表达分布和变化情况。结果免疫组化显示PPARγ在正常大鼠脑内呈弱阳性表达，且主要表达在神经元上;癫持续状态后在星形胶质细胞上呈强阳性表达,神经元形态的细胞上亦有表达。蛋白质印迹分析显示癫持续状态后PPARγ的表达明显增加。结论大鼠癫持续状态后星形细胞上PPARγ表达增加，可能具有神经保护作用，这为癫的神经保护治疗提供了一个新的研究方向。 |
| 关键词: 癫 PPARγ 癫持续状态 脑 星形细胞 |
| DOI：10.3724/SP.J.1008.2010.042 |
| 投稿时间：2009-04-14修订日期：2009-12-14 |
| 基金项目: |
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| Expression of PPARγ in rat brain after status epileticus |
| YU Xin1*,SHAO Xiao-guang2,SUN Hong3,CUI Lian-qi1,HUANG Yuan-gui4 |
| (1.Department of Neurology,No.401 Hospital of PLA,Qingdao 266071,Shandong,China;2.Department of Internal Medicine Ⅱ,The Northern Region of No.401 Hospital,Qingdao 266100,Shandong,China;3.Department of Neurology,the Second Affiliated Hospital of Xi’an Jiaotong University,Xi’an 710004,Shaanxi,China;4.Department of Neurology,Xijing Hospital,Fourth Military Medical University,Xi’an 710032,Shaanxi,China) |
| Abstract: |
| ObjectiveTo observe the expression of PPARγ in rat brain after status epilepticus. MethodsMale Sprague-Dawley rats were randomly divided into a control group and a status epilepticus group(n=10). Lithium-pilocarpine model was used to prepare epileptic model. Immunohistochemical method and Western blotting analysis were used to examine the changes of PPARγ distribution and expression in rat brain in the two groups.ResultsImmunohistochemical staining showed that the expression of PPARγ was weak in the brain of normal rats,and the expression was mainly on the neurons.The expression was strong in astrocytes after status epilepticus,and the expression was also noticed on cells with neuron shape.Western blotting analysis showed that the expression of PPARγ was greatly increased after status epilepticus.ConclusionThe expression of PPARγ is increased on astrocyte after status epilepticus in rats,which may have neuroprotective effect.This finding cast new lights on neuroprotective treatment for epilepsy. |
| Key words: epilepsy PPARγ status epilepticus brain astrocytes |
