| 摘要: |
| 目的:观察雷帕霉素体外对小鼠调节性T细胞(regulatory T cells,Treg)分化和增殖的影响,为进一步探讨其可能的免疫耐受诱导机制奠定基础。方法:无菌条件下取C57BL/6小鼠脾脏,分离单个核细胞,免疫磁珠阴性分选获得CD4+T细胞,分别与0.1 μmol/L雷帕霉素(RAPA组)、0.5 μmol/L环孢素(CsA组)进行共培养7 d,并以正常培养细胞作为空白对照。流式细胞仪检测各组CD4+CD25+Treg细胞比例；RT-PCR检测各组T细胞Foxp3 mRNA表达水平。结果:与空白对照\[(7.42±0.82)%\]相比,CsA组CD4+CD25+Treg细胞\[(3.72±0.74)%\]所占比例明显降低(P<0.01)；RAPA组CD4+CD25+Treg细胞\[(11.47±1.08)%\]所占比例明显升高(P<0.01)。RAPA组T细胞Foxp3 mRNA表达明显高于CsA组和空白对照(P<0.01)；CsA组Foxp3 mRNA表达低于空白对照(P<0.05)。结论:雷帕霉素体外可促进CD4+CD25+ Treg细胞的分化和增殖,有利于免疫耐受的形成,其免疫抑制机制不同于CsA。 |
| 关键词: 调节性T淋巴细胞 雷帕霉素 环孢素A Foxp3 体外 细胞分化 细胞增殖 |
| DOI：10.3724/SP.J.1008.2009.01136 |
| 投稿时间：2009-05-01修订日期：2009-06-10 |
| 基金项目: |
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| Rapamycin promotes differentiation and proliferation of mouse CD4+CD25+ regulatory T cells in vitro |
| XIE Jiang-ping,DING Guo-shan,NI Zhi-jia,FU Hong*,FU Zhi-ren |
| (Department of Organ Transplantation,Organ Transplantation Institute of PLA,Changzheng Hospital,Second Military Medical University,Shanghai 200003,China) |
| Abstract: |
| Objective:To observe the influence of rapamycin on the differentiation and proliferation of CD4+ CD25+ Treg cells,so as to lay a foundation for further studying its role in inducing immune tolerance.Methods: The spleens were taken from C57BL/6 mice under sterile condition and the mononuclear cells were isolated.CD4+ T cells were isolated by immunomagnetic beads (negative) and were divide into normal control group,rapamycin (0.1 μmol/L,for 7 days) group and cyclosporine (0.5 μmol/L,for 7 days) group.Flow cytometry was used to determine the proportions of CD4+CD25+ Treg cells,and RT-PCR was used to examine the expression of Foxp3 mRNA.Results: Compared with the control group,the proportion of CD4+CD25+ Treg cells in the CD4+T cells was significantly decreased (\[7.42±0.82\]% vs \[3.72±0.74\]%,P<0.01) in the cyclosporine group and was significantly increased (\[7.42±0.82\]% vs \[11.47±1.08\]%,P<0.01) in the rapamycin group.Expression of Foxp3 mRNA in rapamycin group was significantly higher than that in the other 2 groups(P<0.01); expression of Foxp3 mRNA in the cyclosporine group was significantly lower than that in the control group (P<0.05).Conclusion: Rapamycin can promote the proliferation and growth of CD4+CD25+Foxp3+Treg cells in vitro, and facilitate the development of immune tolerance.It has a different immune suppression mechanism with cyclosporine A. |
| Key words: regulatory T lymphocytes rapamycin cyclosporine A Foxp3 in vitro cell differentiation cell proliferation |
