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高效液相色谱法同时测定双五胶囊中4种有效成分的含量
叶光明1,姜云云1*,陈云红1,王发祥1,吴明男2,肖飞2
0
(1.解放军101医院药剂科,无锡 214004;2.巢湖市第一人民医院药剂科,巢湖 238000)
摘要:
目的建立同时测定双五胶囊中紫丁香苷、五味子醇甲、五味子甲素、五味子乙素含量的方法。方法HPLC法,采用Diamonsil C18柱(200 mm×4.6 mm,5 μm),流动相为A:甲醇-乙腈(1∶1),B∶水,梯度洗脱,0~5 min, 35%~60%A;5~10 min ,60%~70%A;10~50 min, 70%~90%A;50~90 min, 90%A。流速为1 ml/min,柱温:35℃,检测波长220 nm。结果紫丁香苷、五味子醇甲、五味子甲素、五味子乙素浓度分别在1.28~20.40 μg/ml(r=0.999 7)、6.30~100.80 μg/ml(r=0.999 6)、1.20~19.20 μg/ml (r=0.999 8) 和3.75~60.00 μg/ml(r=0.999 6) 范围内呈良好的线性关系。4种成分精密度实验RSD<1%。24 h 内稳定性RSD<1.5%。紫丁香苷、五味子醇甲、五味子甲素、五味子乙素的平均加样回收率分别为99.47%、102.50%、99.21%、 101.86%。结论所建立的方法具有快速、简便、准确等优点,可用于双五胶囊的质量控制。
关键词:  双五胶囊  紫丁香苷  五味子醇甲  五味子甲素  五味子乙素  高压液相色谱法
DOI:10.3724/SP.J.1008.2010.091
投稿时间:2009-07-26修订日期:2009-11-24
基金项目:
HPLC in simultaneous determination of four components in Shuangwu capsules
YE Guang-ming1,JIANG Yun-yun1*,CHEN Yun-hong1,WANG Fa-xiang1,WU Ming-nan2,XIAO Fei2
(1.Departement of Pharmacy,No.101 Hospital of PLA,Wuxi 214044,Jiangsu,China;2.Department of Pharmacy,the First People’s Hospital of Chaohu City,Chaohu 238000,Anhui,China)
Abstract:
ObjectiveTo establish an HPLC method for simultaneous determination of syringin,schizandrin,deoxyschizandrin and schisandrin B in Shuangwu capsules. MethodsThe HPLC method was employed using a Diamonsil C18 column (200 mm×4.6 mm,5 μm) with a mobile phase of methanol-acetonitrile (1∶1,A) and water (B). The gradient elution program was as follows: 0-5 min,35%-60%A; 5-10 min,60%-70%A; 10-50 min,70%-90%A; 50-90 min,90%A. The flow rate was 1 ml/min. The detection wavelength was set at 220 nm and the temperature was at 35℃.ResultsThe linearity was obtained within the range of 1.28-20.40 μg/ml for syringin (r=0.999 7),6.30-100.80 μg/ml for schisantherin (r=0.999 6),1.20-19.20 μg/ml for deoxyschizandrin (r=0.999 8),and 3.75-60.00 μg/ml for schisandrin B (r=0.999 6). The RSD values of precision were less than 1% for all the four components. The results showed that the samples were stable in the room temperature for at least 24 h. The average recovery rates of syringin,schizandrin,deoxyschizandrin and schisandrin B were 99.47%,102.50%,99.21% and 101.86%,respectively. Conclusion Our method is rapid,easy to perform and accurate; it can be used to control the quality of Shuangwu capsules.
Key words:  Shuangwu capsules  syringin  schizandrin  deoxyschizandrin  schisandrin B  high pressure liquid chromatography