| 摘要: |
| 目的观察地塞米松(Dex)和转化生长因子β1(TGFβ1)对人卵巢癌HO-8910细胞中Ⅰ型纤溶酶原激活物抑制剂(PAI-1)表达的影响,探讨其在卵巢癌的发生发展中可能发挥的作用。方法将HO-8910细胞分别用TGFβ1(0.01~10 ng/ml)和Dex(100 nmol/L)单独或联合处理不同时间后,用实时定量PCR和蛋白质印迹法检测PAI-1 mRNA和蛋白表达的变化；用放线菌素D阻断新的mRNA合成,检测PAI-1 mRNA的稳定性。结果HO-8910细胞中,TGFβ1能以时间和浓度依赖性的方式上调PAI-1 mRNA的表达,最高可上调3.75倍 (P<0.01)；Dex也能上调PAI-1 mRNA的表达,最高为1.65倍 (P<0.01)；Dex和TGFβ1两者联合作用4 h,可上调PAI-1 mRNA 12.7倍 (P<0.01),远大于两者单独作用之和,两者联合处理对PAI-1蛋白的上调作用也大于两者单独作用；Dex和TGFβ1单独及联合作用均不影响PAI-1 mRNA的稳定性。 结论Dex和TGFβ1单独均能上调HO-8910细胞中PAI-1的表达,联用对PAI-1上调有协同作用,该协同作用不是通过增强PAI-1 mRNA的稳定性，而是通过它们在转录水平的作用实现的。 |
| 关键词: 孵巢肿瘤 地塞米松 转化生长因子β1 纤溶酶原激活物抑制物1 |
| DOI：10.3724/SP.J.1008.2010.07 |
| 投稿时间：2009-08-31修订日期：2009-12-02 |
| 基金项目: |
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| Effect of dexamethasone and transforming growth factor β1 on expression of plasminogen activator inhibitor-1 in human ovarian cancer cell line HO-8910 |
| WANG Yan,CAO Dong-mei,WANG Yan,YAN Ya-min,LU Jian* |
| (Department of Pathophysiology,College of Basic Medical Sciences,Second Military Medical University,Shanghai 200433,China) |
| Abstract: |
| ObjectiveTo observe the effect of dexamethasone (Dex) and transforming growth factor β1 (TGFβ1) on the expression of plasminogen activator inhibitor-1 (PAI-1) in human ovarian cancer cell line HO-8910,and to explore its role in the development and progression of human ovarian cancer.Methods Human HO-8910 cells were treated with Dex(100 nmol/L) or TGFβ1(0.01-10 ng/ml) or both for different time periods,then the expression of PAI-1 mRNA and protein was examined by real-time PCR and Western blotting analysis.Act D was used to inhibit the synthesis of new mRNA so as to evaluate the stability of PAI-1 mRNA.ResultsTGFβ1 up-regulated PAI-1 mRNA expression in HO-8910 cells in a time- and dose-dependent manner,with the highest increase being 3.75 folds (P<0.01); Dex also up-regulated PAI-1 mRNA expression,with the highest increase being 1.65 folds (P<0.01).Dex combined with TGFβ1 markedly up-regulated PAI-1 mRNA level,with the highest increase being 12.7 folds (P<0.01),which was greatly higher than the sum of Dex and TGFβ1.Dex combined with TGFβ1 also greatly up-regulated the PAI-1 protein level compared with Dex or TGFβ1 alone.Dex,TGFβ1 or combination of them did not affect the stability of PAI-1 mRNA.ConclusionBoth Dex and TGFβ1 can up-regulate the expression of PAI-1 in HO-8910 cells,and the combination of them can work synergistically to up-regulate PAI-1 expression.The synergistic effect occurs at the transcriptional level,and it is not due to enhenced stability of PAI-1 mRNA. |
| Key words: ovarian neoplasms dexamethasone transforming growth factor β1 plasminogen activator inhibitor 1 |
