| 摘要: |
| 目的结合基因芯片技术、生物信息学分析和染色质免疫共沉淀技术分析小鼠肝再生启动阶段的差异表达基因，并探讨其调控方式。方法采用小鼠全基因组基因表达谱芯片检测并验证肝再生启动阶段2组差异表达基因；应用基于TRANSFAC数据库的PAINT对差异表达基因进行转录调控分析；最后运用染色质免疫共沉淀技术验证NF-κB的潜在靶基因。结果肝切后4 h小鼠差异表达基因共332个(上调127个,下调205个),其中早期反应基因-2(immediate early response 2,IER2)上调约5倍；对所有差异表达基因进行PAINT分析,预测到多个转录因子如Stat家族和NF-κB均参与差异表达基因调控网络；免疫共沉淀技术证实IER2受NF-κB调控。结论NF-κB可能通过调控IER2参与肝再生启动。 |
| 关键词: 微阵列分析 肝再生 IER2 NF-κB |
| DOI：10.3724/SP.J.1008.2010.0123 |
| 投稿时间：2009-09-10修订日期：2009-12-22 |
| 基金项目:国家自然科学基金青年基金(30800553). |
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| Nuclear factor κB participates in liver regeneration through regulating immediate early response 2 |
| CHEN Huan△, XU Qing△, DONG Rui-qi, YANG Sheng-sheng, MIAO Ming-yong, JIAO Bing-hua* |
| (Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Second Military Medical University, Shanghai 200433, China) |
| Abstract: |
| ObjectiveTo investigate the differentially expressed genes and their regulatory network during priming phase of liver regeneration in mice using gene chip technology,bioinformatic analysis,and chromatin immunoprecipitation methods.MethodsLiver tissues of mice from post-hepatectomy and sham groups were processed for microarray to detect the differentially expressed genes.PAINT analysis based on TRANSFAC database was used to analyze the transcription regulatory network for differentially expressed genes.Chromatin immunoprecipitation was employed for the verification of the target genes of NF-κB.ResultsA total of 332 genes were differentially expressed during the priming phase of liver regeneration,with 205 down-regulated and 127 up-regulated.The immediate early response 2(IER2) was increased by about 5 folds.PAINT analysis showed that several transcription factors such as Stat family and NF-κB participated in the regulatory network of these genes.Chromatin immunoprecipitation demonstrated that IER2 was regulated by NF-κB.ConclusionNF-κB participates in priming phase of liver regeneration through regulating IER2. |
| Key words: microarray analysis liver regeneration IER2 NF-κB |
