| 摘要: |
| 绿色荧光蛋白（green fluorescent protein, GFP)是一种来源于水母的生物发光蛋白。GFP基因作为报告基因已被广泛应用于各种生物的研究中。白念珠菌感染在临床真菌感染中占有很大的比例,因此对白念珠菌进行深入研究具有重要意义。GFP在白念珠菌的研究中已得到广泛的应用。GFP基因可与目的基因融合形成融合基因,融合基因可表达融合蛋白,该融合蛋白可发出荧光信号,进行基因表达和蛋白定位等方面的研究。本文综述了GFP在白念珠菌研究中的应用。 |
| 关键词: 绿色荧光蛋白 白念珠菌 基因表达 蛋白定位 |
| DOI：10.3724/SP.J.1008.2010.0541 |
| 投稿时间：2009-09-24修订日期：2009-12-03 |
| 基金项目:国家自然科学基金（30630071, 30870105）. |
|
| Green fluorescent protein in Candida albicans-related studies |
| LU Hui1, CAO Ying-ying2 , WANG Yan1, GAO Ping-hui1, CAO Yong-bing1, JIANG Yuan-ying1* |
| (1. Department of Pharmacology, School of Pharmacy, Second Military Medical University, Shanghai 200433, China;2. Department of Biochemical Pharmacy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China) |
| Abstract: |
| The green fluorescent protein (GFP) is a bioluminescent protein derived from the Jellyfish Aequorea．As a reporter gene, GFP has been used in a variety of biological researches. A large proportion of the clinical fungal infections are caused by Candida albicans, making it very important for further study. GFP has also been widely used in Candida albicans-related studies. The fusion genes harboring GFP gene can yield expression of the fusion protein, which can emit green fluorescence and can be used for gene expression and protein localization. This paper reviews the application of GFP in the Candida albicans-related studies. |
| Key words: green fluorescent protein Candida albicans gene expression protein location |
