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| 氢气饱和生理盐水对大鼠脑缺血再灌注损伤的保护作用 |
| 刘颖1,2,孙强2,张威3,蔡健美3,李润平2*,吕世军1* |
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| (1. 潍坊医学院病理学教研室,潍坊 261042;2. 第二军医大学海医系潜水医学教研室,上海 200433; 3. 第二军医大学长海医院神经内科,上海 200433) |
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| 摘要: |
| 目的研究氢气饱和生理盐水对大鼠局灶性脑缺血再灌注损伤的保护作用及作用机制。方法采用大鼠大脑中动脉栓塞模型(MCAO),24 h后断头取脑,用TTC染色法检测脑组织梗死体积,干湿法测定脑组织含水量,尼氏染色观察大鼠大脑皮质细胞损伤程度,ELISA法测定脑组织内IL-1β、TNF-α含量。结果与对照组相比,氢饱和生理盐水组能够减少MCAO脑梗死体积、减轻皮质区水肿、增加神经元尼氏小体,明显减少脑组织IL-1β和TNF-α含量 (P<0.05)。结论氢气饱和生理盐水能减轻脑组织缺血再灌注损伤程度,其机制可能与其抑制炎症反应有关。 |
| 关键词: 氢气饱和生理盐水 脑缺血 再灌注损伤 |
| DOI：10.3724/SP.J.1008.2010.0238 |
| 投稿时间：2009-10-21修订日期：2010-02-03 |
| 基金项目:国家自然科学基金（30971199），军队“十一五”科技攻关课题（08G066）. |
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| Protective effect of saturated hydrogen saline against cerebral ischemia-reperfusion injury in rats |
| LIU Ying1,2, SUN Qiang2, ZHANG Wei3, CAI Jian-mei3, LI Run-ping2*, L Shi-jun1* |
| (1. Department of Pathology, Weifang Medical College, Weifang 261042, Shandong, China;2. Department of Diving Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, China;3. Department of Neurology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China) |
| Abstract: |
| ObjectiveTo study the protective effect of saturated hydrogen saline against cerebral ischemia-reperfusion injury and the related mechanism. MethodsRat middle cerebral artery occlusion (MCAO) models were established by thread ligation of the middle cerebral artery. The rats were sacrificed 24 h later. The cerebral infarction volume was determined by TTC staining, the water content in brain tissue by dry-wet weight method, the degree of cerebral cells by Nissl staining, and the levels of IL-1β and TNF-α in the ischemic cerebral tissues by ELISA.ResultsCompared with control group, hydrogen saline decreased the brain water content and cerebral infarction volume, and increased the quantity of nissel’s body in the cortex; meanwhile, it also significantly decreased the concentrations of IL-1β and TNF-α in brain tissue(P<0.05).ConclusionHydrogen saline can alleviate the cerebral ischemia-reperfusion injury, probably by inhibiting the inflammation response. |
| Key words: saturated hydrogen saline brain ischemia reperfusion injury |
