| 摘要: |
| 目的应用基因芯片技术筛选人生长激素腺瘤发生、发展相关基因,为后续研究奠定基础。方法应用人全基因组寡核苷酸芯片HG-U133 Plus 2.0检测8例生长激素腺瘤组织、2例混合正常垂体组织的基因表达谱,差异表达基因进行筛选和生物信息学分析。Real-time qPCR验证芯片结果。结果筛选出与生长激素腺瘤相关的上调基因187条,下调基因899条；差异表达基因功能主要涉及分子结合、凋亡或肿瘤相关、代谢、信号转导、细胞周期、物质运输等多个生物过程。结论基因芯片技术可初步筛选出人生长激素腺瘤相关基因；生长激素腺瘤的发生、发展是多基因、多分子、多通路的网络调控过程。 |
| 关键词: 分泌生长激素的脑垂体腺瘤 寡核苷酸序列分析 基因表达谱 |
| DOI：10.3724/SP.J.1008.2010.01109 |
| 投稿时间：2009-12-07修订日期：2010-08-14 |
| 基金项目: |
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| Whole genome oligonucleotide microarrays in screening of growth homone adenoma associated genes |
| GAN Xiao-qiang1, LU Yi-cheng1, HU Guo-han1, CHEN Ju-xiang1, XIAO Hua-sheng2,DING Xue-hua |
| (1. Department of Neurosurgery, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China; 2. National Engineering Center for Biochip at Shanghai, Shanghai 201203, China) |
| Abstract: |
| ObjectiveTo screen for differentially expressed genes associated with the development and progression of human growth hormone adenoma, so as to lay a foundation for future study. MethodsThe whole genome oligonucleotide microarray (Affymetrix 133 plus 2.0) was used to examine gene profiles of 8 growth homone adenoma samples and 2 normal pooled pituitary samples. Differentially expressed genes were subjected to bioinformatics analysis. Real-time qPCR was used to verify the microarray result of a randomly selected candidate gene. ResultsA total of 187 up-regulated genes and 899 down-regulated genes associated with growth hormone adenoma were screened out, with their functions mainly associated with molecular binding, apoptosis/tumor, metabolism, signal transduction, cell cycle, and transportation activities.Conclusion Microarray technology can be used for preliminary screen of growth hormone adenoma associated genes. The development and progression of growth homone adenoma are complex processes involving multiple genes, molecules, and pathways. |
| Key words: growth homone-secreting pituitary adenoma oligonucleotide array sequence analysis gene expression profile |
