| 摘要: |
| 目的观察胃癌组织XAB2基因及蛋白表达的变化,探讨其与胃癌发生发展的可能关系。方法选取34例胃癌患者的肿瘤组织及癌旁正常组织,采用real- time RT-PCR技术检测XAB2 mRNA的表达；采用免疫组化方法检测胃癌组织、癌旁正常组织、胃炎组织XAB2蛋白的表达；分析不同临床病理指标下胃癌组织XAB2蛋白的表达差异。结果胃癌组织XAB2 mRNA表达显著低于癌旁正常组织(P<0.01),且在胃癌进展早期(Ⅰ+Ⅱ期)即明显下降。免疫组化检测显示：胃癌组织中XAB2蛋白表达的阳性率为50.0%(17/34),明显低于配对癌旁正常组织82.4%(28/34)和胃炎组织91.1%(31/34),差异具有统计学意义 (P<0.01),而后二者间无统计学差异。胃癌组织XAB2蛋白表达与胃癌分化程度正相关(P=0.041),与肿瘤浸润深度、肿瘤大小负相关 (P=0.019、0.027)；在不同性别、年龄、肿瘤部位、有无淋巴结转移、TNM分期间无统计学差异。结论XAB2低表达可能与胃癌发生、进展密切相关,值得深入研究以利于临床胃癌诊治。 |
| 关键词: XAB2蛋白 胃肿瘤 组织阵列分析 差异表达 |
| DOI：10.3724/SP.J.1008.2010.0148 |
| 投稿时间：2009-12-09修订日期：2010-01-15 |
| 基金项目:上海市公共卫生“三年行动”计划(08GWZX0201,08GWZX0101),上海市科技创新行动计划(09DZ1950101). |
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| Decreased XAB2 expression and gastric cancer |
| CAI Hui1, CHANG Wen-jun2, HAN Yi-fang2, GU Li-qiang1, MA Li-ye1*, CAO Guang-wen2* |
| (1.Department of General Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433,China;2.Department of Epidemiology, College of Basic Medical Sciences, Second Military Medical University, Shanghai 200433, China) |
| Abstract: |
| ObjectiveTo investigate the changes of XAB2 mRNA and protein expression in gastric cancer tissues, so as to discuss its possible relationship with the development of gastric cancer. MethodsXAB2 mRNA expression in 34 gastric cancer tissues and the matched adjacent normal tissues was examined by real-time RT-PCR. Meanwhile, tissue biochip and immunohistochemistry were employed to examine XAB2 protein expression in gastric cancer tissues, matched adjacent normal tissues, and gastritis tissues. The expression of XAB2 in different tissues and its clinical significance was analyzed. Results XAB2 mRNA expression in the gastric cancer tissues was significantly lower than that in corresponding adjacent normal tissues (P<0.01), and the expression level was greatly decreased at early stage of gastric cancer (stageⅠ+Ⅱ). Immunohistochemical examination showed that the positive rate of XAB2 gene was significantly lower in gastric cancer tissues (50.0%, 17/34) than those in the adjacent tissues (82.4%, 28/34) and gastritis tissues (91.1%, 31/34, P<0.01),with no significant differences found between the latter two tissues. The XAB2 protein expression in gastric cancer tissues was positively correlated to tumor differentiation(P=0.041) and negatively with tumor infiltration and size (P=0.019 and P=0.027, respectively). XAB2 expression was not associated with patients’ sex, age, tumor position, lymph node metastasis or TNM stages(P>0.05). Conclusion The lower XAB2 expression might be closely associated with the development and progression of gastric cancer, and further studying may benefit the diagnosis and therapy of gastric carcinoma patients. |
| Key words: XAB2 protein stomach neoplasms tissue array analysis differential expression |
