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硒化镉量子点对人黑素瘤细胞和正常表皮细胞的毒性
宋方茗,郑红,娄子洋*
0
(第二军医大学药学院药物分析测试中心,上海 200433)
摘要:
目的 通过cell counting kit-8(CCK-8)检测不同粒径硒化镉(CdSe/ZnS)量子点对人恶性黑素瘤细胞A375、A375.s2以及正常人表皮细胞HaCaT的细胞毒性,研究硒化镉量子点对黑素瘤细胞生长的影响。方法 分别取处于对数生长期的A375、A375.s2以及HaCaT细胞铺入96孔板内,次日分别向铺有细胞的孔内加入浓度为162、81、54、40.5、27、10.125、4.05、2.025、1.012 5 nmol/L水溶性量子点(QDs)-605与QDs-545进行孵育,24 h后每孔加入10 μl CCK-8,测定2种硒化镉量子点对A375、A375.s2及HaCaT细胞增殖的影响。结果 CCK-8法显示,随着QDs-605与QDs-545浓度的增加,A375和A375.s2细胞存活率随之下降。不同浓度的QDs-605与QDs-545作用于A375、A375.s2细胞,24 h后其细胞存活率均低于对照组(P<0.05,P<0.01),但是对HaCaT细胞没有抑制作用。结论 硒化镉量子点对A375、A375.s2人黑素瘤细胞有一定的抑制作用,而对HaCaT正常人表皮细胞抑制作用不明显。
关键词:  硒化镉量子点; 黑素瘤; 表皮细胞; 细胞增殖  细胞毒性
DOI:10.3724/SP.J.1008.2010.0465
投稿时间:2010-01-27修订日期:2010-04-21
基金项目:上海市科委纳米技术专项基金(0852nm04200).
Cytotoxic effect of CdSe/ZnS quantum dots on melanoma cells and normal human epidermal cells
SONG Fang-ming, ZHENG Hong, LOU Zi-yang*
(Center of Drug Analysis, School of Pharmacy, Second Military Medical University, Shanghai 200433, China)
Abstract:
Objective To study the cytotoxic effect of the CdSe/ZnS quantum dots of different diameters on human malignant melanoma cell line A375, A375.s2 and normal human epidermal cell line HaCaT using cell counting kit-8(CCK-8), so as to study the influence of CdSe/ZnS quantum dots on growth of melanoma cells. Methods Log phase A375,A375.s2 and HaCaT cells were plated into 96-well plates. After a 24 h incubation, the prepared QDs-605 and QDs-545 solutions of different concentrations (162, 81, 54, 40.5, 27, 10.125, 4.05, 2.025, and 1.012 5 nmol/L) were added into the wells. After another 24 h incubation, CCK-8 was added into the well (10 μl/well).Then the cytotoxicities of CdSe/ZnS quantum dots on the proliferation of A375, A375.s2 and HaCaT cells were determined by MTT assay. Results We found that with the increase of QDs-605 and QDs-545 concentrations, the survival rates of A375, A375.s2 cells were decreased. A day after A375, A375.s2 cells were treated with different concentrations of QDs-605 and QDs-545, the cell survival rate was significantly lower than that of the control group(P<0.05,P<0.01); however, different concentrations of QDs-605 and QDs-545 did not inhibit the proliferation of HaCaT cells.Conclusion CdSe/Zns quantum dots have inhibitory effect on the growth of human malignant melanoma cell line A375, A375.s2, and have no noticeable effect on normal human epidermal cell line HaCaT.
Key words:  Supported by the Nano-technology Foundation of Shanghai Science and Technology Committee (0852nm04200).