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| 正交试验优选仙茅酚苷类成分的提取方法 |
| 刘雷1,2,韩婷2,薛黎明2,王寅3,秦路平2,辛海量4,张巧艳1,2* |
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| (1. 福建中医药大学药学院生药学教研室,福州 350108; 2. 第二军医大学药学院生药学教研室,上海 200433; 3. 解放军455医院药剂科，上海 200052; 4. 第二军医大学中医系,上海 200433) |
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| 摘要: |
| [摘要]目的研究仙茅酚苷类成分的最佳提取方法。方法以苔黑酚葡萄糖苷、仙茅苷、仙茅素3种酚苷类成分的含量为考察指标,以HPLC进行含量测定,采用正交试验确定仙茅酚苷类成分的最佳提取方法。各因素考察水平:乙醇浓度(50%、70%、95%)、提取次数(1、2、3次)、提取时间(1、1.5、2 h)、溶剂用量(6、8、10倍量)。结果乙醇浓度、提取时间、提取次数和溶剂用量均影响仙茅酚苷类成分的提取效率,其中以乙醇浓度的影响最为显著。最佳提取方法为10倍量70%乙醇回流提取3次,每次2 h。结论采用本研究方法能够充分提取仙茅的酚苷类化学成分。 |
| 关键词: 仙茅 酚苷 正交试验 提取法 |
| DOI：10.3724/SP.J.1008.2010.01244 |
| 投稿时间：2010-02-03修订日期：2010-09-16 |
| 基金项目:国家自然科学基金(81073115),上海市基础研究重点项目(09JC1417600). |
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| Optimization of extraction process for phenolic glycosides from Rhizoma Curculigins by orthogonal test |
| LIU Lei1,2, HAN Ting2, XUE Li-ming2, WANG Yin3, QIN Lu-ping2, XIN Hai-liang4, ZHANG Qiao-yan1,2* |
| (1. Department of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350108, Fujian, China; 2. Department of Pharmacy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China; 3. Department of Pharmaceutical, No. 455 Hospital of PLA, Shanghai 200052, China; 4. Department of Traditional Chinese Medicine, Second Military Medical University, Shanghai 200433, China) |
| Abstract: |
| [Abstract]ObjectiveTo optimize the extraction process for phenolic glycosides from Rhizoma Curculigins. MethodsThe contents of orcinol glucoside,curculigoside and curculigine were used as parameters, and they were determined by HPLC. The extraction conditions of phenolic glycosides from Rhizoma Curculigins were optimized using orthogonal design. The examined factors included concentration of ethanol(50%, 70%, and 95%), extraction time (1, 1.5, and 2 h), times of extraction (1, 2, and 3), and solvent volume (6, 8, and 10 times).MethodsThe concentration of ethanol, extraction time, times of extraction and solvent volume significantly affected the extraction efficacy of phenolic glycosides in Rhizoma Curculigins, with the most prominent effect exerted by the ethanol concentration. The optimal extraction process was refluxing three times with 70% ethanol (10 times of solvent volume), for 2 h each time. MethodsThe present method can effectively extract phenolic glycosides from Rhizoma Curculigin, which paves a way for further research on phenolic glycosides in Rhizoma Curculigins. |
| Key words: Curculigo orchioides phenolic glycosides orthogonal test extraction |
