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白花丹参上调Bcl-2抑制内皮细胞凋亡
于长凯1,2,张晓艳2,高允生1*
0
(1.泰山医学院药学院药理学教研室,泰安 271016; 2.江苏联合职业技术学院连云港中医药分院,连云港 222006)
摘要:
[摘要]目的研究白花丹参对过氧化氢(H2O2)诱导的人脐静脉血管内皮细胞(HUVEC)凋亡的抑制作用及对凋亡相关基因Bcl-2表达的调节。方法应用酶消化灌注法分离人脐静脉血管内皮细胞,采用形态学观察和Ⅷ因子抗体免疫荧光检测法进行鉴定。取对数生长期的细胞分组进行干预(白花丹参高剂量组0.10 g/ml, 白花丹参低剂量组0.01 g/ml),应用流式细胞技术观察细胞凋亡情况,应用免疫细胞荧光技术检测凋亡相关基因Bcl-2的表达。结果在白花丹参干预下,H2O2诱导的HUVEC凋亡率降低(高剂量组P<0.01,低剂量组P<0.05),高剂量组Bcl-2的表达增加(P<0.01)。结论白花丹参对H2O2诱导的HUVEC凋亡具有抑制作用,这种作用与Bcl-2表达上调有关。
关键词:  白花丹参  脐静脉;内皮细胞  细胞凋亡  bcl-2基因
DOI:10.3724/SP.J.1008.2010.01211
投稿时间:2010-03-05修订日期:2010-10-12
基金项目:山东省教育厅科研基金(J04E07).
Salvia miltiorrhiza bge. f. alba up-regulates Bcl-2 and inhibits apoptosis of human umbilical vein endothelial cells
YU Chang-kai1,2, ZHANG Xiao-yan2, GAO Yun-sheng1*
(1. Department of Pharmacology, School of Pharmacy, Taishan Medical University, Taian 271016, Shandong, China; 2. Lianyungang Branch of Traditional Chinese Medicine, Jiangsu Union Technical Institute, Lianyungang 222006, Jiangsu, China)
Abstract:
[Abstract]ObjectiveTo study the influence of Salvia miltiorrhiza bge.f.alba(SMA) on apoptosis and Bcl-2 expression in human umbilical vein endothelial cell (HUVEC) in vitro.MethodsHUVECs were isolated using perfusion and enzyme digestion methods, and the obtained cells were identified by morphological observation and ⅧAg immunoreactivity examination. The cells in the exponential phase of growth were treated with H2O2 and different concentrations of SMA(high dose: 0.10 g/ml, low dose: 0.01 g/ml). The cell apoptosis was determined by flow cytometric analysis and Bcl-2 expression was examined by immunofluorescence mehtod. MethodsSMA significantly decreased the H2O2-induced apoptosis of HUVECs (P<0.01 in high dose group and P<0.05 in low dose group), and significantly increased the expression of Bcl-2 in high dose group(P<0.01). MethodsSMA can inhibit H2O2-induced apoptosis of HUVEC, which might be associated with the increase of Bcl-2 expression.
Key words:  Salvia miltiorrhiza bge.f.alba  umbilical veins  endothelial cell  apoptosis  bcl-2 genes