| 摘要: |
| 目的 分析钾通道KCNQ1在不同发育阶段大鼠胰腺以及成年大鼠胰岛中的表达情况,为探悉其在胰腺B细胞发育及功能中可能的作用提供实验依据。方法 采用高密度寡核苷酸芯片对胚胎第12.5天(E12.5)、E15.5、E18.5、新生和成年大鼠胰腺进行基因转录水平分析,并用RT-PCR对KCNQ1通道亚基在上述5个时期大鼠胰腺以及成年大鼠胰岛中的表达进行验证和分析。结果 与胰腺内分泌细胞分化、成熟有关的转录因子分别于E15.5、E18.5达到表达高峰；E18.5胰腺中B细胞功能成熟标志基因的表达显著增加,而KCNQ1钾离子通道的编码基因KCNQ1及KCNE1此时才出现表达。成年胰腺及胰岛中均具有多种KCNQ1通道亚基的表达。结论 KCNQ1通道的表达出现于胰腺发育后期内分泌细胞功能逐渐成熟阶段,在成年胰岛中亦具有较高表达水平,提示KCNQ1通道可能表达于成熟B细胞并参与其内分泌功能。 |
| 关键词: KCNQ1钾通道； 胰岛； 胰腺 发育 |
| DOI：10.3724/SP.J.1008.2010.0472 |
| 投稿时间：2010-03-17修订日期：2010-04-22 |
| 基金项目:国家自然科学基金(30770537). |
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| Expression of KCNQ1 channel during rat pancreas development |
| LIU Li-jie1, SHI Li-juan1, WANG Li1, GUO Jing2, DE Wei2 |
| (1. Department of Physiology, Medical College of Southeast University, Nanjing 210009, Jiangsu, China;2. Department of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing 210029, Jiangsu, China) |
| Abstract: |
| Objective To investigate the dynamic expression profile of KCNQ1 channel in rat developing pancreas and adult rat islets, so as to provide experimental basis for further analyzing the possible role of KCNQ1 channel in β-cell development and function. Methods The gene expression patterns of embryonic day 12.5 (E12.5), E15.5, E18.5, new-born and adult rat pancreas were compared using the GeneChipRAE 230A. The expressions of KCNQ1 channel subunits in rat pancreas at the above five stages and adult rat islets were also detected and analzyed by RT-PCR. Results The results of genechip showed that the expression of transcription factors related to endocrine cell differention and maturation peaked at E15.5 and E18.5, respectively. The expression of mature β-cell specific genes increased significantly at E18.5, but KCNQ1 and KCNE1 only started to express at E18.5. The results of RT-PCR showed expression of multiple KCNQ1 channel subunits in adult rat pancreas and islet. Conclusion The expression of KCNQ1 channel appears in the later stage of pancreatic development when the endocrine cells undergo further maturation, and has a higher level in adult islets. The expression profile of KCNQ1 channel subunits indicates that KCNQ1 channel might be expressed in mature β-cell and involved in its endocrine function. |
| Key words: KCNQ1 potassium channel islets of Langerhans pancreas development |
