| 摘要: |
| 目的探讨microRNA分子miR-203对食管鳞癌Eca109细胞增殖及侵袭能力的影响。方法根据人源miR-203序列,设计并合成其双链模拟物。通过脂质体转染将miR-203模拟物分子导入食管鳞癌Eca109细胞中,转染无关microRNA模拟物作为对照。测定2组细胞的细胞倍增时间、细胞凋亡率以及侵袭细胞率,观察miR-203对Eca109细胞增殖及侵袭能力的影响。结果Eca109细胞转染miR-203后其细胞倍增时间为(26.1±0.5) h,较对照组(24.2±0.6) h明显延长(P<0.01);其凋亡细胞比例较对照组增加\[(4.5±0.4)% vs (3.7±0.4)%,P<0.05\];Transwell小室侵袭实验显示转染miR-203模拟物后,该组的侵袭细胞率明显低于对照组\[(39.2±5.8)% vs (49.5±6.8)%,P<0.05\]。结论miR-203能抑制Eca109细胞的增殖和侵袭能力,提示miR-203可能是食管鳞癌生物治疗的潜在靶点。 |
| 关键词: 微小RNA miR-203 食管鳞癌 细胞增殖 细胞侵袭 |
| DOI：10.3724/SP.J.1008.2010.0818 |
| 投稿时间：2010-04-23修订日期：2010-04-23 |
| 基金项目:国家自然科学基金(30872552), 上海市自然科学基金(10140902300). |
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| MiR-203 inhibits proliferation and invasion of esophageal squamous cell carcinoma |
| YUAN Yang, GONG De-jun, LIU Xiao-hong,,LU Fang-lin* |
| (Department of Cardiothoracic Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China) |
| Abstract: |
| ObjectiveTo investigate the influence of miR-203 on the proliferation and invasion of human esophageal squamous cell carcinoma cell line Eca109. MethodsDouble-stranded mimics of miR-203 were designed and transfected into Eca109 cells with Lipofectamine 2000; Eca109 cells transfected with nonsense microRNA mimics were taken as control. The proliferation ability of Eca109 cells was determined by calculating the cell population doubling time and the percentage of apoptotic cells; the invasion ability of Eca109 cells was determined by Transwell assay. ResultsIn vitro experiment showed that, compared with the control group, Eca109 cells transfected with miR-203 mimics had a significantly longer cell population doubling time (\[26.1±0.5\] h vs \[24.2±0.6\] h, P<0.01), a higher cell apoptotic rate (\[4.5±0.4\]% vs \[3.7±0.4\]%, P<0.05), and a lower cell invasion rate (\[39.2±5.8\]% vs \[49.5±6.8\]%,P<0.05). ConclusionOur data shows that miR-203 can inhibit the proliferative and invasive abilities of Eca109 cells, suggesting that miR-203 might be a potential gene therapeutic target of human esophageal squamous cell carcinoma. |
| Key words: microRNA miR-203 esophageal squamous cell carcinoma proliferation invasion |
