| 摘要: |
| \[摘要\]目的观察乌饭树叶醇提物及其不同溶剂的萃取物对睡眠剥夺所致大鼠脑干抗氧化能力的影响。方法采用小站台水环境的方法对大鼠进行睡眠剥夺,给予高、中、低剂量(分别相当于生药含量7.2、2.4、1.2 g/ml)乌饭树叶醇提物及其醇提物不同萃取物连续灌胃10 d,每天1 ml,以红景天苷(200 mg/ml)作为阳性对照,观察不同剂量的乌饭树叶醇提物及其不同萃取物对大鼠脑干丙二醛(MDA)、超氧化物歧化酶(SOD)和总抗氧化能力(T-AOC)含量的影响。结果正常对照组MDA、SOD和T-AOC分别为(16.4±0.42) nmol/mg protein、(2.9±0.62) U/mg protein、(154.3±14.47) U/ml。与正常对照组相比,睡眠剥夺模型组大鼠脑干MDA、SOD和T-AOC含量均升高(P＜0.05),阳性对照组红景天苷和高剂量醇提物组以及乌饭树叶水提取物组MDA含量减少(P＜0.05),SOD和T-AOC也降低。结论高剂量乌饭树叶醇提物可以降低脑干的氧化应激水平,具有较强的抗氧化能力,其活性部位主要位于水提物内。 |
| 关键词: 乌饭树 脑干 氧化性应激 睡眠剥夺 |
| DOI：10.3724/SP.J.1008.2011.0172 |
| 投稿时间：2010-05-04修订日期：2011-01-17 |
| 基金项目:全军医学科学技术研究“十一五”计划课题 (06MA168). |
|
| Effect of Vaccinium bracteatum Thunb. Leaf ethanol extract on anti-oxidative capability of brain stem in sleep-deprived rats |
| MA Wen-ling, HUANG Li-na, GUO Jun-sheng |
| (Department of Military Hygiene,Faculty of Navy Medicine, Second Military Medical University,Shanghai 200433,China) |
| Abstract: |
| \[Abstract\]ObjectiveTo investigate the effects of Vaccinium bracteatum Thunb. Leaf ethanol extract(VEE)and its different extracts on the anti-oxidative capability of brain stem in sleep-deprived rats. MethodsRats were intragastrically administered with low, middle, and high dose of VEE (1.2 g/ml, 2.4 g/ml, and 7.2 g/ml)and different extracts daily for 10 days. The rat mental fatigue models were established by sleep deprivation through flower-pot on the 7th day. Rats treated with salidroside (200 mg/ml)were taken as positive controls. After 72 h sleep deprivation, the concentrations of malondialdehyde (MDA), superoxide dismutase (SOD), and total antioxidative capacity(T-AOC) in the brain stem were determined in each group. ResultsThe concentrations of the MDA, SOD, and T-AOC in the normal control group were (16.4±0.42) nmol/mg protein, (2.9±0.62) U/mg protein, and (154.3±14.47) U/ml, respectively. Compared with the normal control group, the sleep-deprived group had significantly higher concentrations of MDA, SOD and T-AOC(P＜0.05), and the groups of salidroside, high dose ethanol, and aqua extract of VEE had significantly lower MDA (P<0.05), and lower SOD and T-AOC.Conclusion The high dose VEE can greatly decrease the oxidative stress level of the brain stem, and it also has remarkable anti-oxidative ability, with the active part mainly in the aqua extract of VEE. |
| Key words: Vaccinium bracteatum Thunb. brain stem oxidative stress sleep deprivation |
