| 摘要: |
| 目的研究中国南海硬棘软珊瑚Scleronephthya sp.生物活性成分。方法利用正、反相硅胶柱层析、Sephadex LH20以及HPLC等层析手段对一种硬棘软珊瑚中的生物活性成分进行分离。应用波谱分析技术并结合文献数据,对所得的化合物进行结构鉴定。采用琼脂扩散试验对分离得到的化合物进行体外抗微生物活性测试。结果和结论从该种硬棘软珊瑚中分离得到3个过氧化麦角甾醇(1～3)、鲛肝醇(4)、鲨肝醇(5)和1,2O十八烷基甘油缩醛(6)。初步体外抗微生物活性筛选发现这些化合物均有不同程度的抗菌活性。化合物5对大肠杆菌和巨大芽孢杆菌具有一定的抑制活性,化合物6对花药黑粉菌具有很强的抑制活性。化合物1～4仅显示微弱的抗微生物活性。 |
| 关键词: 硬棘软珊瑚 分离和提纯 分子结构 抗真菌药 |
| DOI：10.3724/SP.J.1008.2011.021 |
| 投稿时间：2010-06-18修订日期：2010-12-02 |
| 基金项目:国家自然科学基金面上项目(30873200), 上海市重点基础研究项目(08JC1405600), 上海市重点学科建设项目(B906), 上海市浦江人才项目(PJ2008). |
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| Study on bioactive constituents of the South China Sea soft coral Scleronephthya sp. |
| HUO Juan,TANG Hua,LI Ling,LIU Bao-shu,SUN Peng,YI Yang-hua*,ZHANG Wen* |
| (Research Center for Marine Drugs, School of Pharmacy, Second Military Medical University, Shanghai 200433, China) |
| Abstract: |
| ObjectiveTo investigate the bioactive constituents of coral Scleronephthya sp. collected from the South China Sea. MethodsThe compounds were isolated and purified using repeated column chromatographies on Sephadex LH20, normal and reversedphase silica gels, and RPHPLC. The structures of the compounds were elucidated based on the detailed spectroscopic analysis in combination with reported data. The in vitro antimicrobial activity of these compounds were assessed by an agar diffusion test. Results and conclusion Three 5α,8αepidioxysterols, two alkyl glycerol ethers and 1,2Oalkyl glycerol were isolated from Scleronephthya sp.；these compounds displayed different levels of antifungal and antibacterial activities in bioassay in vitro. Compound 5 inhibited the growth of Gramnegative bacterium Escherichia coli and the Grampositive bacterium Bacillus megaterium. Compound 6 showed marked antifungal activity against Microbotryum violaceum. Compounds 14 demonstrated a weak antimicrobial activity. |
| Key words: Scleronephthya sp. isolation&purification; molecular structure antifungal agents |
