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| 胰岛素样生长因子1对PC12细胞胱硫醚-β-合成酶和硫化氢的影响及机制 |
| 孟涛1,晏勇1*,张华1,晏宁2,代政伟1,李洁颖1,赵丰丽1,彭竹芸1,易洋3 |
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| (1. 重庆医科大学附属第一医院神经内科,重庆 400016;2. 重庆医科大学附属第二医院神经内科,重庆 400010; 3. 武警安顺支队卫生队,安顺 561000) |
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| 摘要: |
| \[摘要\]目的观察胰岛素样生长因子1(IGF-1)对大鼠嗜铬细胞瘤细胞(PC12)胱硫醚-β-合成酶(CBS)及硫化氢(H2S)表达的影响以及可能的细胞信号通路机制。方法用不同剂量IGF-1(20、 40、80 ng/ml)作用体外培养的PC12细胞24 h,荧光定量PCR检测CBS基因表达,蛋白质印迹分析检测CBS、细胞外信号调节激酶/丝裂原活化蛋白激酶(ERK/MAPK)相关蛋白表达,敏感硫电极法检测H2S气体含量。之后选取最佳剂量的IGF-1(80 ng/ml) 作用于30 min前已加入ERK/MAPK抑制剂PD98059的PC12细胞再次检测CBS、ERK/MAPK相关蛋白及H2S气体表达水平。结果IGF-1增加CBS的表达和H2S的含量,上调pERK1/2的表达；而PD98059能够抑制IGF-1的上述作用。结论IGF-1及其调控的ERK/MAPK信号通路参与调节CBS及H2S的表达。 |
| 关键词: 胰岛素样生长因子1 丝裂原活化蛋白激酶类 胱硫醚-β-合成酶 硫化氢 |
| DOI：10.3724/SP.J.1008.2010.01296 |
| 投稿时间：2010-06-21修订日期：2010-11-21 |
| 基金项目:重庆市医学科研计划项目(2010-1-17). |
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| Effects of insulin-like growth factor-1 on cystationine-β-synthase and hydrogen sulfide in PC12 cells |
| MENG Tao1, YAN Yong1*, ZHANG Hua1, YAN Ning2, DAI Zheng-wei1, LI Jie-ying1, ZHAO Feng-li1, PENG Zhu-yun1, YI Yang3 |
| (1. Department of Neurology, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China;2. Department of Neurology, the Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China;3. Health Unit, Chinese People’s Armed Police Forces Anshun Branch, Anshun 561000, Guizhou, China) |
| Abstract: |
| \[Abstract\]ObjectiveTo investigate the effect of insulin-like growth factor-1 (IGF-1) on expression of cystationine-β-synthase(CBS)and hydrogen sulfide(H2S) in rat adrenal pheochromocytoma cell line (PC12) and the possible signaling pathway. MethodsAfter PC12 cells were treated with 20,40, or 80 ng/ml IGF-1 for 24 h,fluorecence quantitative PCR was used to examined the expression of CBS gene, Western blotting analysis was used to detect the expression of CBS,ERK/MAPK protein expression, and sensitive sulphur electrode was used to analyze the levels of H2S gas. For inhibition studies,25 or 50 mol/L PD98059,a specific ERK/MAPK inhibitor,was used 30 min prior to 80 ng/ml IGF-1 treatment; and the above parameters were examined again using the same method. ResultsIGF-1 increased the expression of CBS,pERK1/2, and the levels of H2S；and PD98059 could inhibit the above effect of IGF-1.ConclusionIGF-1 and its ERK/MAPK signaling pathway regulate the expression of CBS and levels of H2S. |
| Key words: insulin-like growth factor-1 mitogen-activated protein kinases cystationine-β-synthase hydrogen sulfide |
