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ZBTB20基因敲除小鼠出生后肝脏甲胎蛋白基因持续高表达的病理学意义
张海1,谢志芳1,ClaudeSzpirer2,,章卫平1*
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(1.第二军医大学基础部病理生理学教研室,上海 200433;2.比利时布鲁塞尔大学生物与分子医学研究所,12 Rue Profs Jeneer & Brachet, B-6041 Gosselies, Belgium)
摘要:
目的 探讨出生后肝脏甲胎蛋白(AFP)基因异常高表达在ZBTB20基因敲除小鼠生长发育障碍、严重低血糖和未成年死亡等表型中可能的病理作用。方法 将ZBTB20单基因敲除小鼠与AFP单基因敲除小鼠杂交,建立ZBTB20与AFP的联合基因敲除小鼠模型,观察并监测其与ZBTB20单基因敲除小鼠在生长发育、存活率及血糖水平上的差异。结果 ZBTB20/AFP双基因敲除小鼠繁殖成功后,通过观察和监测发现其在生长发育、生存率及血糖代谢等方面与ZBTB20单基因敲除小鼠相比无明显差异。结论 ZBTB20单基因敲除小鼠生长发育障碍、严重低血糖和未成年死亡等表型与其AFP基因出生后异常开放无显著关系。
关键词:  甲胎蛋白类  ZBTB20  锌指蛋白  基因敲除小鼠
DOI:10.3724/SP.J.1008.2010.01045
投稿时间:2010-08-16修订日期:2010-09-28
基金项目:国家自然科学基金(90608026,30772478).
Pathological implication of postnatally up-regulated AFP expression in ZBTB20 knockout
ZHANG Hai1, XIE Zhi-fang1, Claude Szpirer2,,ZHANG Wei-ping1*
(1. Department of Pathophysiology, College of Basic Medical Sciences, Second Military Medical University, Shanghai 200433, China;2. Universit Libre de Bruxelles, Institut de Biologie et de M decine Mol culaires, 12 Rue Profs Jeneer & Brachet, B-6041 Gosselies, Belgium)
Abstract:
Objective To investigate the pathological roles of postnatally up-regulated alpha-fetoprotein(AFP) expression in ZBTB20 knockout mice in postnatal growth retardation, metabolic dysfunction, and pre-mature mortality. Methods ZBTB20 and AFP knockout mice were generated by crossbreeding ZBTB20 knockout mice with AFP knockout mice.The postnatal growth, survival rate, and blood glucose of ZBTB20/AFP double knockout mice were observed and compared with those of ZBTB20 knockout mice. Results There were no significant differences in postnatal growth, survival, and glucose homeostasis between ZBTB20/AFP doube knockout mice and ZBTB20 knockout mice. Conclusion The postnatally up-regulated liver AFP in ZBTB20 knockout mice is not associated with the growth retardation, hypoglycemia, or pre-mature mortality.
Key words:  alpha-fetoproteins  ZBTB20  zinc finger protein  knockout mice