| 摘要: |
| 目的探讨不同放射剂量全身放射对小鼠切割伤口愈合撕裂强度的影响。方法采用 60Coγ 射线一次性均匀照射4、6或8 Gy,照后30 min 内,全层切开小鼠背部正中皮肤,构建不同剂量全身放射+切割复合伤动物模型(n=20),以小鼠单纯切割伤作为对照组 (n=10)。各组动物于伤后第10天分别处死,取伤口处全层皮肤组织,利用生物力学方法分析伤口皮肤组织撕裂强度,并结合组织病理学方法综合评价伤口愈合情况。结果复合伤组小鼠伤口随照射剂量增加,撕裂强度降低明显。10 d时,4 Gy组伤口撕裂强度为(114.26±0.29) g,与单纯切割伤组\[(117.12±1.86) g\]相比差异无统计学意义,而6 Gy组\[(91.87±1.96) g\]和8 Gy组伤口撕裂强度 \[(55.26±2.64) g\]均低于单纯切割伤组(P<0.05)。HE 染色显示,与单纯切割伤组相比，复合伤组小鼠伤口胶原纤维排列无序,组织疏松,成纤维细胞增殖较少。结论放射损伤延缓伤口愈合,全身放射+切割复合伤时伤口撕裂强度随放射剂量增高而降低。 |
| 关键词: 全身照射 撕裂强度 伤口愈合 |
| DOI：10.3724/SP.J.1008.2011.09 |
| 投稿时间：2010-10-09修订日期：2010-12-28 |
| 基金项目:上海市自然科学基金(09ZR1410600). |
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| Total body γ-irradiation decreases wound breaking strength during wound healing in rats |
| WANG Guo-dong1,WANG Liang1,BAI Shu-bo1,LIU Xin-yuan2,WU Yang1* |
| (1. Department of Stomatology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China;;2. Hygiene Division, Department of Administrative Affairs, Second Military Medical University, Shanghai 200433, China) |
| Abstract: |
| ObjectiveTo investigate the influence of total body irradiation with different doses of 60Coγ on wound breaking strength during wound healing in rats. MethodsRats were exposed to 60Coγ radiation at dosages of 4,6,and 8 Gy. Within 30 min after irradiation, full thickness skin wounds were made on the shaved back of rats to establish animal models of irradiatedtrauma injury plus skin wounds (n=20), and nonirradiated rats with pure incision injury were used as controls (n=10). The rats were sacrificed at day 10 after treatment, and the full thickness skin wounds were harvested. Biomechanics method and histopathology examination were used to evaluate the wound breaking strength and histological features after healing. ResultsThe wound breaking strength of model groups were greatly retarded with the increase of irradiation doses. Statistical results showed that on day 10 the wound breaking strength values were significantly different between 4 Gy group ([114.26±0.29] g) and control group ([117.12±1.86] g, P>0.05), and the wound breaking strength values of 6 Gy group ([91.87±1.96] g) and 8 Gy group ([55.26±2.64] g) were significantly lower than that of the control group (P<0.05). HE staining showed that the wounds in the irradiation trauma groups had looser and disorder collagenous fiber and less fibroblast proliferation than control group. ConclusionRadiation injury may result in delayed wound healing, and the wound breaking strength decreases with the increase of irradiation dose in model of irradiation injury plus full thickness skin wounds. |
| Key words: whole-body irradiation breaking strength wound healing |
