| 摘要: |
| 目的建立含量测定方法,对拉曼光谱内标法中2种参量的定量分析结果进行比较,确定拉曼内标定量法最适合的参量。方法分别以拉曼峰的相对峰强和峰面积比值作为参量,以不同采收时期和不同产地的连翘叶为对象,采用内标法进行连翘苷的含量测定。以相对峰强为参量时,选取样品溶液拉曼光谱中连翘苷苯环振动(1 319.1 cm-1处的强峰)和溶剂甲醇中CH3O反对称伸缩振动(2 974.7 cm-1)作为定量峰和内标参比峰；以峰面积比值作为参量时,选取连翘苷苯环振动(1 319.1 cm-1处的强峰)和溶剂甲醇中CH3O对称伸缩振动(3 020.9 cm-1)作为定量峰和内标参比峰。分别以定量峰与内标峰强和峰面积的比值为纵坐标,以连翘苷浓度为横坐标绘制标准曲线,并对线性和加样回收率进行比较。结果以2种参量作为响应值用于拉曼光谱定量分析,均有良好的线性,相关系数r分别为0.998 0和0.997 6; 回收率均在100.04%～101.30%之间,回收完全；对2种参量的测定结果进行线性拟合,得出回归方程C2=1.030 4C1-0.033 1, r=0.999 6。结果表明两者测得结果一致,利用2种参量进行定量分析都能取得准确结果。结论无论以相对峰强,还是以峰面积比值作为参量都可以用于拉曼光谱定量分析,2种参量的测定结果基本一致。拉曼光谱用于连翘苷的定量分析具有操作简便、迅速等优点,可以用于中草药的含量测定。 |
| 关键词: 药物分析 含量测定 拉曼光谱分析 连翘苷 |
| DOI：10.3724/SP.J.1008.2011.062 |
| 投稿时间：2010-10-15修订日期：2010-12-03 |
| 基金项目: |
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| Raman spectroscopy in determination of forsythin content |
| WANG Wei*,XI Xin-xin,YANG Hao,ZHOU Shao-hua,JING Heng-cui,LI Jian-bo |
| (Department of Pharmacy, Pharmaceutical College of Henan University, Kaifeng 475004, Henan, China) |
| Abstract: |
| ObjectiveTo compare the two parameters in Raman spectroscopy quantitative analysis, so as to confirm the optimal one. MethodsThe ratios of peak intensity and peak area were taken as parameters in quantitative analysis. The contents of forsythin in forsythia suspense leaves gathered at different time points from different regions were analyzed with laser Raman spectroscopy. When the ratio of the peak intensity was taken as parameter, the Raman peak of forsythin (ρArH [1 319.1 cm-1]) and the peak of methanol (νas CH3O [2 974.7 cm-1]) were selected as quantitative peak and internal standard reference peak in the confocal microRaman spectra of forsythin methanol solution. When the ratio of the peak area was taken as parameter, the Raman peak of forsythin (ρArH [1 319.1 cm-1]) and the peak of methanol (νsCH3O[3 020.9 cm-1]) were selected. The ratios of intensity and area were taken as ordinate, and the content of forsythin was taken as abscissa. The two standard curves were plotted and the linear and recoveries were compared. ResultsBoth parameters had good linear relationship with forsythin concentration, with the correlation coefficients being 0.998 0 and 0.997 6; the recoveries were 100.04%101.30% and the recycling was complete. The linear fitting of the results with two parameters were measured, and the regression equation was C2=1.030 4C1-0.033 1, r=0.999 6. The results obtained with the two parameters were accurate and were largely identical. ConclusionBoth the relative peak intensity and the ratio of the peak area can be used as parameters for forsythin quantitative analysis with Raman spectroscopy. And the two parameters can obtain largely identical results. Raman spectroscopy with internal standard is a simple and rapid method for quantitative analysis of forsythin, and it can be used for quantitative analysis for Chinese herbs. |
| Key words: pharmaceutical analysis quantitative analysis Raman spectrum analysis forsythin |
