| 摘要: |
| 中药和天然产物在生物体内通常发生广泛代谢，因其代谢途径的复杂性和多组分、多靶点，整体调节作用的特点，现已将代谢组学应用于中药代谢产物及其药效机制的研究中。但是，由于代谢组学研究要求获得生物体液中大量外源性与内源性代谢物信息，这就对分析技术提出了很高的要求。本文综述了中药代谢物和代谢组学研究中常用的液质联用技术，并对Pubmed检索到的23篇相关文献进行数据归纳与比较分析，重点讨论不同种类液质联用技术在中药代谢组学研究应用中的优缺点，指出UPLC-Q-TOF/MS的优势，以期为中药代谢组学研究提供新思路。 |
| 关键词: 中药 代谢产物 代谢组学 液质联用 |
| DOI：10.3724/SP.J.1008.2011.0658 |
| 投稿时间：2010-11-11修订日期：2011-03-29 |
| 基金项目: |
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| Application analysis of LC-MS techniques in traditional Chinese medicine metabolites and metabonomics researches |
| ZHANG BIN,LOU Zi-yang* |
| (\[Acad J Sec Mil Med Univ,2011,32(6):658-662\]) |
| Abstract: |
| For the complexity of the metabolic pathways and the characteristics of “multiple components, multiple targets and the overall adjustment functions” of Traditional Chinese medicine (TCM) and natural products in organism, nowadays, metabonomics have been widely applied in TCM metabolites and pharmacodynamic mechanism researches. However, the study of metabonomics needs much exogenous and endogenous metabolites information of biological fluids, which requires more powerful analytical techniques. The paper reviewed liquid chromatography coupled with mass spectrometry (LC-MS) techniques commonly applied to TCM metabolites and metabonomics researches. And then we summarized and comparatively analyzed the data of 23 literatures searched from Pubmed database. The advantages and disadvantages of different kinds of LC-MS techniques in TCM metabonomics studies were discussed, and the advantages of UPLC-Q-TOF/MS were indicated, which contributes to provide new ideas for TCM metabonomics researches. |
| Key words: TCM metabolites metabonomics LC-MS |
