摘要: |
G蛋白偶联受体(G protein-coupled receptor, GPCR)是一个重要的细胞膜受体超家族。β-arrestin 1和β-arrestin 2是广泛存在于细胞内的调配器和支架蛋白,它们在G蛋白偶联受体的细胞内信号转导、脱敏、内化、复敏以及G蛋白非依赖的信号转导中发挥重要作用。生物发光共振能量转移(BRET)技术能够实时监测活细胞内GPCRs和β-arrestins的相互作用,可进一步阐明β-arrestin调节GPCRs的作用机制,有助于开发新一代影响GPCRs功能活动的药物。 |
关键词: β-arrestin G蛋白偶联受体 信号转导 生物发光共振能量转移 |
DOI:10.3724/SP.J.1008.2011.01140 |
投稿时间:2011-01-04修订日期:2011-04-15 |
基金项目:国家自然科学基金 |
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Application of BRET technology in interaction of G protein-coupled receptor with β-arrestins: recent progress |
DU Hui1,2,BAI Bo3*,CHEN Jing3 |
(1. Department of Neurobiology, College of Basic Medical Sciences, Taishan Medical College, Taian 271000, Shandong, China 2.Department of Life Science, Shandong Agricultural University, Taian 271000, Shandong, China 3. Department of Neurobiology, Jining Medical College, Jining 272000,Shandong, China *Corresponding author.) |
Abstract: |
G Protein-coupled receptors constitute a super family of cell surface proteins. β-arrestin 1 and 2 are widely expressed intracellular adaptor and scaffolding proteins that play important roles in G protein-coupled receptor desensitization, internalization, intracellular traf?cking and G Protein-independent signalling. Recent developments in bioluminescence resonance energy transfer (BRET)technology enable monitor the GPCR–β-arrestin complexes in live cells and real time. In concert with a range of experimental tools, investigators can elucidate the ever-expanding roles of β-arrestins in mediating GPCR function, which will be benefit for developing new generation drugs targeting GPCRs. |
Key words: β-arrestin G protein-coupled receptor signaling transduction bioluminescence resonance energy transfer BRET |