| 摘要: |
| 目的探讨影响原发性肾透明细胞癌(clear cell renal cell carcinoma,ccRCC)根治术患者的预后与肿瘤转移相关基因CD99的关系及预后的影响因素。方法应用半定量RT-PCR技术研究CD99基因在ccRCC组织和癌旁组织中的表达,通过随访调查患者的预后、生存时间及其危险因素,应用Cox风险回归模型分析获得影响预后的主要危险因素。结果与对应癌旁组织相比,CD99基因在ccRCC组织中表达上调的占73.5％,差异有统计学意义(P=0.000)。应用Cox风险回归模型分析发现:ccRCC组织中CD99基因的高表达\[HR=0.14, 95%CI(0.01,2.15)\]与根治术患者预后的生存时间无关；而患者年龄\[HR=1.18, 95%CI(1.01,1.38)\]、TNM分期\[HR=51.91,95%CI(4.31,625.87)\]、合并糖尿病\[HR=59.94,95%CI(2.21,1 627)\]和合并高血压\[HR=47.72,95%CI(1.37,1 670)\]是影响患者预后生存时间的主要危险因素。比较TNMⅠ期和Ⅱ~Ⅳ期两组患者的1年生存率和2年生存率(100.0% vs 60.0%,P=0.004；93.8% vs 8.3%,P=0.000),差异有统计学意义。结论肿瘤转移相关基因CD99的表达可能与ccRCC的预后无关,年龄、TNM分期、合并糖尿病和合并高血压是影响患者预后生存时间的主要危险因素。 |
| 关键词: CD99 基因表达 肾肿瘤 透明细胞癌 预后 |
| DOI：10.3724/SP.J.1008.2011.0517 |
| 投稿时间：2011-02-11修订日期：2011-04-17 |
| 基金项目:上海市宝山区科学技术委员会基金(09-E-10). |
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| Relationship between prognosis of patients with primary clear cell renal cell carcinoma after radical nephrectomy and expression of CD99 and analysis of prognostic factors |
| CAO Ting-hu1,WANG Lei2,KOU Yu-bin1,HU Jin-xin1,ZHU Jun1,HOU Jian-guo3* |
| (1. Department of Urology, Baoshan Branch of Shanghai Shuguang Hospital,Shanghai University of Traditional Chinese Medicine, Shanghai 201900, China; 2. Department of Clinical Diagnosis, Zhangjiagang Hospital of Traditional Chinese Medicine, Zhangjiagang 215600, Jiangsu, China; 3. Department of Urology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China) |
| Abstract: |
| ObjectiveTo investigate the relationship between prognosis of patients with primary clear cell renal cell carcinoma (ccRCC) after radical nephrectomy and expression of tumor metastasis-associated gene CD99 and to analyze the prognostic factors of ccRCC patients. MethodsSemi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) was used to examine the expression of CD99 in primary ccRCC tissues and their corresponding adjacent tissues. The prognosis and risk factors of survival time of patients were studied by follow-up investigation, and the main risk factors were screened by Cox hazard regression model. ResultsCompared with the adjacent renal tissues, 73.5% ccRCC tissues had up-regulated CD99 expression, with significant difference found between the two groups( P=0.000). Cox hazard regression model showed that high CD99 expression in ccRCC tissues was not a survival risk factor of ccRCC patients after radical nephrectomy (HR=0.14, 95%CI\[0.01,2.15\]); and age (HR= 1.18, 95%CI\[1.01,1.38\]), TNM stages (HR=51.91, 95%CI\[4.31,625.87\]), diabetes (HR=59.94, 95%CI\[2.21,1 627\]) and hypertension (HR=47.72, 95%CI\[1.37,1 670\]) were the major risk factors for the survival of patients after radical nephrectomy. The 1-year and 2-year survival rates of ccRCC patients in TNM stage Ⅰ were significantly higher than those in TNM stage Ⅱ-Ⅳ, respectively (100.0% vs 60.0%, P=0.004; 93.8% vs 8.3%,P=0.000). ConclusionThe expression of tumor metastasis-related gene CD99 may not be associated with the prognoses of ccRCC patients. Age, TNM stage, diabetes and hypertension are the major risk factors of prognosis after resection of ccRCC. |
| Key words: CD99 gene expression kidney neoplasms clear cell carcinoma prognosis |
