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骨关节炎患者关节软骨及滑膜中MMP-9、IL-6的表达
郭静1,闫冰2,李琪佳1*,甘洪全3,王志强3
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(1. 河北联合大学医学实验研究中心,唐山 063000;2. 河北联合大学附属医院血液科,唐山 063000;3. 河北联合大学附属医院骨科,唐山 063000)
摘要:
目的探讨骨关节炎中基质金属蛋白酶9(MMP-9)、白介素6 (IL-6) mRNA和蛋白的表达。方法采用免疫组织化学技术检测60例骨关节炎病例(OA组)及20例正常对照关节软骨及滑膜MMP-9、IL-6蛋白的表达水平;采用原位分子杂交技术检测30例OA及10例正常对照关节软骨及滑膜MMP-9、IL-6 mRNA的表达水平。结果MMP-9 mRNA和蛋白在OA组阳性表达的积分光密度(IOD)值均高于正常对照组\[(8.38±2.29) vs (3.52±1.36),P<0.01;(9.18±3.58) vs (3.83±1.48),P<0.01\],OA中二者的表达正相关性(r=0.924,P<0.01);IL-6 mRNA和蛋白在OA组阳性表达的IOD值均高于正常对照组\[(8.74±3.62) vs (5.91±1.53),P<0.01;(8.96±3.57) vs (6.87±1.43),P<0.01\],OA中二者的表达亦正相关(r=0.917,P<0.01);MMP-9 mRNA、IL-6 mRNA及相应蛋白在OA中的表达均正相关(原位杂交r=0.426,P<0.05;免疫组化r=0.444,P<0.01)。结论MMP-9、IL-6在OA关节软骨与滑膜中呈高表达,二者对OA的发生发展可能起促进作用。
关键词:  骨关节炎  基质金属蛋白酶9  白介素6  原位杂交  免疫组织化学
DOI:10.3724/SP.J.1008.2011.0672
投稿时间:2011-03-02修订日期:2011-04-21
基金项目:河北省人事厅留学归国人员科技活动基金(200911).
Expression of metalloproteinase-9 and interleukin-6 in articular cartilage and synovium of osteoarthritis patients
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Abstract:
[Abstract] Objective To investigate the significance of expression of MMP-9, IL-6 mRNA and corresponding proteins in Osteoarthritis. Methods Immunohistochemical(IHC) analysis was used to determine the distribution of MMP-9 and IL-6 protein in chondrocytes and synoviocytes of osteoarthritis from 60 patients with osteoarthritis group and 20 patients with normal control group. Expression of MMP-9 and IL-6 mRNA in chondric and synovial tissues was detected in 30 cases with osteoarthritis group and 10 cases with normal control group by in situ hybridization(ISH). Results IOD of MMP-9 mRNA and corresponding protein in OA was significantly higher than normal control group (P<0.01), and there was difference between IOD of IL-6 mRNA and corresponding protein in OA and control group (P<0.05). There were positive correlations both between mRNA and corresponding protein in OA (r=0.924, 0.917; P<0.01). There were also significantly positive correlations both between IOD of MMP-9 and IL-6 in OA detected by ISH and IHC (r=0.426, P<0.05; r=0.444, P<0.01),respectively. Conclusion IL-6 may be up-regulate the expression of MMP-9 of articular chondrocytes and synoviocytes in OA, and both promote the biological behavior of OA such as occurrence,progress,etc.
Key words:  osteoarthritis  metalloproteinase-9  interleukin-6  in situ hybridization  immunohistochemistry