| 摘要: |
| 目的观察上皮性卵巢癌细胞株及肿瘤组织microRNA-200c(miR-200c)的表达水平,探讨其可能的临床价值。方法采用Real-time RT-PCR技术比较人卵巢癌母细胞系(HO-8910)、卵巢癌高转移能力变异细胞株(HO-8910PM)及HO-8910细胞团簇中miR-141、miR-200c的表达差异。同法测定上皮性卵巢癌(n=83)、交界性卵巢肿瘤(n=13)及正常卵巢组织(n=8)中miR-141、miR-200c的表达,并分析卵巢癌不同临床病理特征下二者的表达差异。结果与HO-8910、HO-8910PM 相比,HO-8910细胞团簇中miR-200c表达下调(P＜0.05)；正常卵巢组织、卵巢交界性肿瘤、卵巢癌中miR-141、miR-200c表达依次上调(P＜0.05)。转移性卵巢癌、低分化卵巢癌及透明细胞卵巢癌中miR-200c表达下调（P<0.05）。miR-200c高表达患者预后优于miR-200c低表达者(P＜0.05)。结论miR-200c表达下调与卵巢癌进展相关,可能提示卵巢癌预后不良。 |
| 关键词: 微RNAs 卵巢肿瘤 预后 |
| DOI：10.3724/SP.J.1008.2011.0612 |
| 投稿时间：2011-03-04修订日期：2011-05-09 |
| 基金项目: |
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| Changes of miRNA-200c expression in ovarian cancer and its clinical significance |
| LI Lin-xia1,LI Shuang-di1,YANG Yi-xia1,WAN Xiao-ping2* |
| (1. Department of Obstetrics and Gynecology, First People’s Hospital, Shanghai Jiaotong University, Shanghai 200080, China;2. Department of Gynecology, International Peace Maternity & Child Health Hospital, Shanghai 200030, China) |
| Abstract: |
| ObjectiveTo observe the expression of microRNA-200c (miR-200c) in ovarian cancer cell line and tissues, and to assess its possible clinical value. MethodsReal-time PCR was used to analyze the expression of miR-200c and miR-141 in HO-8910 (human ovarian cancer cell line), HO-8910PM (highly metastatic ovarian cancer cell line), and HO-8910 cluster cells. We also determined the expression of miR-200c and miR-141 in 83 ovarian cancer tissues, 13 borderline ovarian tumors, and 8 normal ovary tissues; the expression was compared between tumors of different pathological characters. ResultsExpression of miR-200c was down-regulated in HO-8910 cluster cells compared with those in HO-8910 and HO-8910PM cells(P＜0.05).Expressions of miR-141 and miR-200c were gradually up-regulated from the normal ovary tissue to borderline ovarian tumors, then to ovarian cancer tissues (P<0.05). Expression of miR-200c was down-regulated in the metastatic ovarian cancer and ovarian clear cell tumors and undifferentiated ovarian cancer cells(P<0.05). Patients with high miR-200c expression had a better prognosis than those with a low expression of miR-200c (P<0.05). Conclusion The decreased expression of miR-200c is correlated with the progression of ovarian cancer patients, and it is a risk factor of poor prognosis. |
| Key words: microRNAs ovarian neoplasms prognosis |
