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| 儿童急性淋巴细胞白血病β-arrestin1表达升高的临床意义 |
| 刘慧1,2,龙娟1,谭俊杰1,孙滨3,张鹏辉1,邹琳1* |
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| (1. 重庆医科大学附属儿童医院临床分子医学中心,重庆 400014,2.重庆医科大学附属第一医院输血科,重庆 400016;3. 重庆医科大学附属第二医院检验科,重庆 400010) |
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| 摘要: |
| 目的探讨β-arrestin1在儿童急性淋巴细胞白血病(ALL)中的表达及临床意义。 方法收集155例初发ALL患儿的骨髓样本(ALL组),分离单个核细胞,以51例非恶性血液疾病患者作为对照(Ctrl组),将ALL患者分成高危、中危和标危组,采用实时荧光定量RT-PCR法检测β-arrestin1 mRNA的表达,采用蛋白质印迹法和免疫荧光法检测β-arrestin1蛋白表达的变化。用Spearman统计分析β-arrestin1表达与ALL患者临床和生物学特点之间的相关性。 结果ALL组β-arrest |
| 关键词: 急性淋巴细胞白血病 β-arrestin1 单个核细胞 |
| DOI：10.3724/SP.J.1008.2011.0701 |
| 投稿时间：2011-03-15修订日期：2011-06-01 |
| 基金项目:国家自然科学基金(90919013, 30871103)；重庆市自然科学基金（2008BB5072，2010BA5008）；教育部“新世纪优秀人才支持计划”(NCET-2008) |
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| Up-regulation of β-arrestin1 in children with acute lymphoblastic leukemia and its clinical significance |
| LIU Hui1,LONG Juan1,TAN Jun-jie1,SUN Bin3,ZHANG Peng-hui1,ZOU Lin1* |
| (1. Center for Clinical Molecular Medicine, Children’s Hospital of Chongqing Medical University, Chongqing 400014, China; 2. Department of Blood Transfusion, The First Affiliated Hospital of Chongqing Medical University,Chongqing 400016, China;3. Department of Laboratory Medicine, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China) |
| Abstract: |
| Objective To explore the expression and clinical significance of β-arrestin1 in newly-diagnosed childhood acute lymphoblastic leukemia (ALL). Methods One hundred and fifty-five newly-diagnosed childhood ALL patients were enrolled in this study. Fifty-one sex- and age-matched patients without malignant hematological diseases were used as the control. The bone marrow samples were collected, then mononuclear cells of these samples were isolated. The mRNA expression of β-arrestin1 were detected by using quantitative real-time RT-PCR, and the protein expression were detected by western blot and immunofluorescence assays. The Spearman Correlation Coefficient was used to analyze the correlations between β-arrestin1 expression and ALL patients’s clinical and biological features. Results The expression of β-arrestin1 was significantly up-regulated in ALL patients compared with that in controls (P<0.01). β-arrestin1 expression was significantly higher in high risk (HR) group and medium risk (MR) group than that in standard risk (SR) group of ALL, as well as β-arrestin1 expression was higher in HR group than that in MR group. Moreover, β-arrestin1 expression was positively correlated with WBC count and risk classification of ALL with Spearman correlation analysis. Conclusion β-arrestin1 expression is correlated with the risk classfication of ALL, which could be a useful marker for the diagnosis, therapy strategy and prognosis. |
| Key words: Leukemia lymphocyte β-arrestin1 mononuclearcell |
