| 摘要: |
| 目的 对比观察雄激素依赖前列腺癌细胞(LNCaP)与雄激素非依赖前列腺癌细胞(LNCaP-AI)中microRNA的表达差异，探讨前列腺癌激素依赖向非依赖转化的转录后调控机制。方法 利用Agilent基因芯片检测LNCaP及LNCaP-AI细胞microRNA的表达，用RT-PCR方法对其中6个microRNA进行验证，并对差异表达的microRNA所调控靶基因功能进行生物信息学分析。结果 基因芯片检测发现：与LNCaP 相比，LNCaP-AI细胞有27个microRNA表达降低，11个microRNA表达升高。对其中6个microRNA进行RT-PCR验证，结果与基因芯片结果一致。通过检索http://www.mirbase.org/针对microRNA调控的靶基因，并参考已知的与雄激素非依赖相关基因的功能，发现LNCaP细胞转化为激素非依赖的LNCaP-AI细胞过程中，差异表达的microRNA主要调控表皮生长因子受体(EGFR)、基质金属蛋白酶9(MMP-9)、Bcl-2及雄激素相关代谢酶。结论 前列腺癌激素非依赖转化过程中存在microRNA的差异表达，可能涉及雄激素受体(AR)旁路信号通路、金属酶、抗凋亡基因及雄激素相关代谢酶基因等。 |
| 关键词: 前列腺肿瘤 雄激素非依赖 LNCaP细胞 微RNAs |
| DOI：10.3724/SP.J.1008.2013.0024 |
| 投稿时间：2012-07-25修订日期：2011-05-31 |
| 基金项目: |
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| MicroRNA alteration associated with prostate cancer LNCaP cell progression to androgen-independence |
| JI Jia-tao,XU Chuang-liang,YE Hua-mao,ZHOU Tie,TANG Yuan-jie,SUN Ying-hao* |
(Department of Urology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
*Corresponding author.) |
| Abstract: |
| Objective To compare profiles of microRNA between the LNCaP and LNCaP-AI cell lines, so as to futher elucidate the post-transcritional mechanism regulating the progression to androgen-independence. Methods The microRNA profiles of LNCaP and LNCaP-AI cell lines were examined by Agilent’s microassay. The expression of six microRNAs was verified by RT-PCR. The functions of differentially expressed microRNAs were eludicated by a search with miRBase software (http://www.mirbase.org/). Results The Aglint’s microRNA microassay showed that 11 microRNAs were up-regulated and 27 were down-regulated during the LNCaP progression to androgen-independence. RT-PCR results were consistent with those of the Agilent’s microassay chips. By searching the targets of microRNAs in the miRBase software, we found that the differentically expressed microRNAs were mainly involved in regulation of matrix metalloproteinase 9 (MMP-9), Bcl-2, and epithelial growth factor receptor (EGFR) and genes mediating androgen metabolism. Conclusion There is alteration of microRNA during the progression of LNCaP to androgen-independence, which may involve androgen receptor related pathway, metalloenzyme, anti-apoptotic gene and genes related to androgen metabolism. |
| Key words: prostatic neoplasms androgen-independent LNCaP cells microRNAs |
