| 摘要: |
| 目的观察富勒烯衍生物C3对小鼠电离辐射的防护效果。方法富勒烯C3(100 mg/kg)腹腔注射后,利用LD50和LD90剂量60Co γ射线对BALB/c小鼠进行全身均匀照射,观察小鼠照后30 d生存率、死亡动物平均存活时间、照后小鼠体内血清氧化指标\[如超氧化物歧化酶(SOD)、谷胱甘肽(GSH)和丙二醛(MDA)\]变化情况、照后30 d外周血白细胞计数情况,以及照后小鼠外源性脾集落形成单位(CFU-S)和体外粒-单系细胞集落形成单位(CFU-GM)计数情况。结果富勒烯衍生物C3可提高γ射线照后小鼠生存率(P<0.01),但照后死亡动物平均存活时间无延长；C3可提高照后小鼠体内血清SOD及GSH浓度(P<0.01),减少照后血清MDA含量(P<0.01)；C3还可提高照后小鼠外周血白细胞计数水平(P<0.01),并且提高照后小鼠外源性CFU-S和体外CFU-GM数量(P<0.01)。结论水溶性富勒烯衍生物C3对小鼠γ射线辐射损伤具有较好的防护作用。 |
| 关键词: 羧酸富勒烯 C3 γ射线 自由基 辐射防护 |
| DOI：10.3724/SP.J.1008.2011.0354 |
| 投稿时间：2011-04-06修订日期：2011-04-11 |
| 基金项目:国家自然科学基金(81072241,81001221). |
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| Protective effects of carboxyfullerene C3 against γ-ray irradiation injury in mice |
| CUI Jian-guo,CHENG Ying,GAO Fu,SUN Ding,ZHOU Chuan-feng,JIANG Hao,CAI Jian-ming,LI Bai-long* |
| (Department of Radiation Medicine, Faculty of Navy Medicine, Second Military Medical University, Shanghai 200433, China) |
| Abstract: |
| ObjectiveTo investigate the protective effects of carboxyfullerene C3 against γ-ray irradiation-induced injury in BALB/c mice. MethodsBALB/c mice were intraperitoneally injected with carboxyfullerene C3 (100 mg/kg) 0.5 h before whole-body γ-ray irradiation at 6.0 Gy (LD50) and 7.2 Gy (LD90), with the dose rates being 1.0 Gy/min. The survival rate, average survival period of dead animals, and leukocyte count were observed 30 days after irradiation. The serum concentrations of superoxide dismutase (SOD), glutathione(GSH), and malondialdehyde (MDA) were examined by ELISA 4 h after γ-ray irradiation. The numbers of exogenous spleen colony-forming units (CFU-S) and in vitro colony-forming units of granulocyte and monocyte (CFU-GM) in mice receiving different regimens of γ-ray irradiation were calculated under microscope. ResultsCarboxyfullerene C3 significantly increased the survival rate of mice on the 30th day after irradiation(P<0.01), but it failed to prolong the average survival time of the dead animals after irradiation. Carboxyfullerene C3increased the serum levels of SOD and GSH(P<0.01), and decreased serum MDA level(P<0.01). Besides, carboxyfullerene C3 also significantly increased the peripheral leukocyte count(P<0.01), and promoted the CFU-S and CFU-GM counts(P<0.01). ConclusionWater-soluble carboxyfullerene C3 has satisfactory protective effect against γ-ray irradiation-induced injury in mice. |
| Key words: carboxyfullerene C3 gamma rays free radicals radiation protection |
