| 摘要: |
| 目的 从侧群(side population,SP)表型细胞具有干细胞特性的角度出发,对肝癌细胞系中的SP细胞进行检测、分离和评价。方法 利用SP细胞能将荧光染料Hoechst33342泵到细胞外的特性,结合流式细胞技术进行分离纯化。结果 在肝癌细胞系PLC、Huh7、Hep3B、SMMC7721及1例临床肝癌组织中检测到SP细胞的比例依次为0.8%、0.3%、0.6%、0.3%、0.4%;未在HepG2中检测到SP细胞。NOD-SCID荷瘤实验发现SP细胞较非SP细胞具有更高的成瘤性。结论 肝癌细胞系及标本组织含有少量的SP细胞,这些SP细胞比非SP细胞具有更强的成瘤性,为进一步分析SP细胞的肿瘤干细胞特性奠定了基础。 |
| 关键词: 肝肿瘤 肝细胞癌 肿瘤干细胞 侧群细胞 肿瘤发生 Hoechst33342 |
| DOI：10.3724/SP.J.1008.2011.01126 |
| 投稿时间：2011-04-25修订日期：2011-08-03 |
| 基金项目:国家自然科学基金面上项目(30600333). |
|
| Enrichment and tumorigenicity identification of cells with side population phenotype from hepatocellular carcinoma in NOD-SCID mice |
| WANG Xin,HE Ya-qin,YANG Wen,WANG Hong-yang* |
(International Co-operation Laboratory on Signal Transduction, Eastern Hepatobiliary Surgery Institute, Second Military Medical University, Shanghai 200438, China
*Corresponding author.) |
| Abstract: |
| Objective To identify, isolate, and evaluate cells with side population phenotype (SP, with the property of stem cells) from hepatocellular carcinoma. Methods Capable of pumping fluorochrome Hoechst33342 outside of cells, SP cells were isolated and purified by flow cytometry. Results We found that the ratios of SP cells in PLC,Huh7,Hep3B,SMMC7721 cell line and an HCC specimens were 0.8%, 0.3%,0.6%,0.3%,and 0.4%, respectively. We did not find SP cells in HepG2. NOD-SCID subcutaneous transplant experiments showed that SP cells had a high ability of tumorigenicity than non-SP cells. Conclusion Hepatocellular carcinoma cell lines and specimens contain a small number of SP cells, which have a higher tumorigenicity than non-SP cells; this finding paves a way for further analysis of the stem-like property of SP cells. |
| Key words: liver neoplasms hepatocellular carcinoma neoplastic stem cells side population tumorigenesis Hoechst33342 |
